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Journal of Neuroscience, Vol 12, 2819-2837, Copyright © 1992 by Society for Neuroscience
Intracellular recordings from neurobiotin-labeled cells in brain slices of the rat medial nucleus of the trapezoid body
MI Banks and PH Smith
Neuroscience Training Program, University of Wisconsin, Madison 53706.
Principal cells in the medial nucleus of the trapezoid body (MNTB) are
believed to be critical components in the circuit subserving sound
localization. These cells, located in the superior olivary complex, convert
excitatory inputs, arriving from the contralateral cochlear nucleus by way
of large somatic synapses (the calyces of Held), to inhibitory projections
onto principal cells in the ipsilateral lateral superior olive (LSO). We
have characterized a population of cells in the rat MNTB using
intracellular recording and labeling techniques in a brain slice
preparation. MNTB principal cells had spherical or ellipsoid somata that
gave rise to single large-diameter dendrites, which branched extensively
and often extended beyond the borders of MNTB. Commonly observed axonal
projection targets included LSO, the superior paraolivary nucleus, and the
medial superior olive, and occasionally the lateral nucleus of the
trapezoid body. The projections of individual MNTB cells showed an orderly
topography that is consistent with the known tonotopic maps of the nuclei.
In response to current injection, principal cells exhibited several
nonlinearities, including rectification for depolarizing currents and a
"sag" in the membrane potential for hyperpolarizing currents.
Superthreshold depolarizing currents elicited transient firing behavior.
Application of the potassium channel blocker 4-aminopyridine reduced or
eliminated the rectification in the current-voltage relationships and
caused depolarizing currents to elicit repetitive firing. Stimulation of
afferent inputs elicited short-latency spikes, presumably driven by
calyceal synaptic inputs; long-latency, presumably polysynaptic, EPSPs; and
short- and long-latency IPSPs. The duration of synaptic events was strongly
dependent on membrane potential, and this effect was probably due to the
intrinsic membrane properties of the cell. In all cases tested, EPSPs were
blocked by CNQX or DNQX, and IPSPs were blocked by strychnine. Two injected
non-principal cells differed from principal cells in their morphologies and
physiological characteristics.
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