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Journal of Neuroscience, Vol 12, 2888-2900, Copyright © 1992 by Society for Neuroscience
Differential and transient expression of GABAA receptor alpha-subunit mRNAs in the developing rat CNS
MO Poulter, JL Barker, AM O'Carroll, SJ Lolait and LC Mahan
Laboratories of Neurophysiology, NINDS, NIH, Bethesda, Maryland 20892.
The expression of mRNAs coding for alpha 1, alpha 2, alpha 3, alpha 5, and
alpha 6 subunits of the GABAA neurotransmitter receptor was followed during
the development of the rat CNS by in situ hybridization histochemistry.
Expression of these subunit mRNAs in tissue sections of embryonic day 15
and 17 (E15, E17) whole rat and in brain at ages greater than E17 to adult
were varied, transient, and region specific. Subunit mRNAs first detected
at E15 were those coding for the alpha 2 and alpha 3 subunits. At E17,
alpha 2, alpha 3, and alpha 5 mRNAs were present in abundance in numerous
areas in the CNS, with lower but significant amounts of alpha 6 being
present in the cortical neuroepithelial layers. However, alpha 6 subunit
mRNA expression in the cortex declined until little or no alpha 6 mRNA was
detected at E19. alpha 1 subunit mRNA first appeared at E19 in the cortex,
followed by expression in the hippocampus by postnatal 5 (PN5).
Particularly high expression of alpha 2 and alpha 5 subunit mRNAs was
detected throughout the developing CNS, but they were most abundant in the
olfactory bulb neurons. The high levels of alpha 2 and alpha 5 subunit
mRNAs began to decline around PN5 to the amounts observed in adult. These
results demonstrate that numerous GABAA receptor alpha-subunits are
expressed before birth in a region- and age-specific manner. This complex
and varied expression supports the hypothesis that GABA may play a role in
cellular and synaptic differentiation.
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