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Journal of Neuroscience, Vol 12, 3392-3398, Copyright © 1992 by Society for Neuroscience
A novel N18TG2 x mesencephalon cell hybrid expresses properties that suggest a dopaminergic cell line of substantia nigra origin
GD Crawford Jr, WD Le, RG Smith, WJ Xie, E Stefani and SH Appel
Department of Neurology, Baylor College of Medicine, Houston, Texas 77030.
A dopaminergic neuroblastoma was derived using somatic cell fusion of rat
embryonic mesencephalon cells and the murine neuroblastoma-glioma cell line
N18TG2. The resulting interspecies hybrid, named MES23.5, has retained a
stable phenotype and karyotype for a continuous culture period of 1 year.
The hybrid exhibits several properties that suggest that the parent primary
neurons originated in the substantia nigra. The cell line contains tyrosine
hydroxylase, which is identifiable both by biochemical and immunological
methods and synthesizes dopamine, but no other catecholamine. Additionally,
the cell line expresses apparent voltage-gated CA2+ channels as measured by
high-affinity omega- conotoxin binding. The MES23.5 omega-conotoxin
receptors are of similar affinity class to those found in adult rat
mesencephalon. No dihydropyridine receptors, as measured by PN200-100
ligand binding, are present. None of these properties are found in the
N18TG2 parent. At least three neuronal features, namely, tyrosine
hydroxylase, dopamine synthesis, and omega-conotoxin receptor expression,
are quantitatively elevated after sustained treatment with cAMP analogs.
The cell line expresses a complex range of neural properties found in the
dopaminergic neurons of the substantia nigra, and may therefore be useful
elucidating further details of their cell biology.
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