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Journal of Neuroscience, Vol 13, 127-143, Copyright © 1993 by Society for Neuroscience
Growth cone dynamics during the migration of an identified commissural growth cone
PZ Myers and MJ Bastiani
Department of Biology, University of Utah, Salt Lake City 84112.
We have used time-lapse video microscopy to study the behavior of a neuron,
Q1, that pioneers the posterior commissure of the embryonic grasshopper.
Our goal is to use time-lapse video as a tool to acquire a precise picture
of normal development over time, and thereby identify stereotypic
activities that might indicate important interactions necessary for proper
formation of the commissure. We have identified specific and reproducible
behaviors that suggest the presence of underlying cellular interactions
that may play a role in pathfinding. In particular, the Q1 growth cone
undergoes several morphological changes as it contacts the midline. As a
commissural neuron, the midline may be a target in its outgrowth; Q1's
typical response upon contacting the midline with its filopodia, however,
is a rapid retraction. This inhibitory reaction can be overridden by
contact with filopodia of its contralateral homolog. Q1's growth cone can
translocate across the midline at an accelerated rate by a process
resembling "filopodial dilation" (O'Connor et al., 1990) once the two Q1
growth cones meet. Ablation of the contralateral Q1 blocks Q1's advance
across the midline. We have also analyzed in detail the behavior of
individual filopodia to identify behavioral differences that could indicate
differences in substrate adhesivity. Except for instances of filopodial
dilation seen only at the midline, we found no significant asymmetries in
rates of filopodial extension and retraction, or in the survival times of
individual filopodia. We suggest that either the adhesive signal used by Q1
is relatively weak, requiring the integration of many adhesive interactions
by many filopodia to be resolved, or the guidance cues may not be adhesive
in nature.
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