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Journal of Neuroscience, Vol 13, 442-454, Copyright © 1993 by Society for Neuroscience
Three subtypes of alpha-bungarotoxin-sensitive nicotinic acetylcholine receptors are expressed in chick retina
KT Keyser, LR Britto, R Schoepfer, P Whiting, J Cooper, W Conroy, A Brozozowska- Prechtl, HJ Karten and J Lindstrom
Department of Neurosciences, University of California, San Diego 92093- 0608.
A recent report described the isolation of cDNA clones encoding alpha 7 and
alpha 8 subunits of alpha-bungarotoxin-sensitive nicotinic ACh receptors
(alpha BgtAChRs) from chick brain and demonstrated that they were related
to, but distinct from, the alpha subunits of nicotinic ACh receptors
(nAChRs) from muscles and neurons. Monoclonal antibodies against the two
alpha BgtAChR subunits were used to demonstrate that at least two subtypes
are present in embryonic day 18 chicken brain. The predominant brain
subtype contains alpha 7 subunits, while a minor subtype contains both
alpha 7 and alpha 8 subunits. Both subtypes may also contain other
subunits. Here we report the results of immune precipitation studies and
immunohistochemical studies of alpha BgtAChRs in the chick retina. In
addition to the two subtypes found in brain, a new alpha BgtAChR subtype
that contains alpha 8 subunits, but not alpha 7 subunits, was identified
and was found to be the major subtype in chick retina. This subtype has a
lower affinity for alpha-bungarotoxin (alpha Bgt) than does the subtype
containing only alpha 7 subunits. Small amounts of this alpha 8 subtype
were also detected in brain by labeling with higher concentrations of
125I-alpha Bgt than had been used previously. The subtype containing only
alpha 7 subunits comprised 14% of the alpha BgtAChRs in hatchling chick
retina. The subtype containing alpha 8 subunits (but no alpha 7 subunits)
accounted for 69%, and the alpha 7 alpha 8 subtype accounted for 17%.
Amacrine, bipolar, and ganglion cells displayed alpha 8 subunit
immunoreactivity, and a complex pattern of labeling was evident in both the
inner and outer plexiform layers. In contrast, only amacrine and ganglion
cells exhibited alpha 7 subunit immunoreactivity, and the pattern of alpha
7 subunit labeling in the inner plexiform layer differed from that of alpha
8 subunit labeling. These disparities suggest that the alpha BgtAChR
subunits are differentially expressed by different populations of retinal
neurons. In addition, the distribution of alpha BgtAChR subunit
immunoreactivity was found to differ from that of alpha-Bgt- insensitive
nAChR subunits.
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|
|
|
|
|
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|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
|
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Science,
September 22, 1995;
269(5231):
1692 - 1696.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M.-C. Hernandez, L. Erkman, L. Matter-Sadzinski, T. Roztocil, M. Ballivet, and J.-M. Matter
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J. Biol. Chem.,
February 17, 1995;
270(7):
3224 - 3233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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Target-independent diversification and target-specific projection of chemically defined retinal ganglion cell subsets
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January 11, 1995;
121(11):
3763 - 3776.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Fucile, E. Palma, A. M. Mileo, R. Miledi, and F. Eusebi
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March 28, 2000;
97(7):
3643 - 3648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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April 15, 2002;
540(2):
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|
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