QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by De Koninck, P. Articles by Cooper, E. Search for Related Content PubMed PubMed Citation Articles by De Koninck, P. Articles by Cooper, E.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 13, 577-585, Copyright © 1993 by Society for Neuroscience

ARTICLE

NGF induces neonatal rat sensory neurons to extend dendrites in culture after removal of satellite cells

P De Koninck, S Carbonetto and E Cooper
Department of Biology, McGill University, Montreal, Quebec, Canada.

Vertebrate sensory neurons have a pseudo-unipolar morphology; their somata are covered by satellite cells and lack dendrites or synaptic contacts. However, when neonatal rat sensory neurons from the nodose ganglia develop in culture in absence of satellite cells and with NGF, they form synapses among themselves. In this study, we investigated whether neonatal rat nodose neurons express dendrites under the same culture conditions. We show by Lucifer yellow injection that nodose neurons remain typically unipolar when cocultured with their ganglionic satellite cells. However, when these neurons are cultured without satellite cells, virtually all neurons acquire a multipolar morphology. Moreover, when NGF is added to satellite cell-free cultures, several neurons extend dendrites; these processes stain positively for microtubule-associated protein-2. NGF induces a 17-fold increase in dendritic outgrowth after 3 weeks but has little effect on axon number. In addition, we find that the ability of nodose neurons to extend dendrites is developmentally regulated. Furthermore, in a combined morphological and electrophysiological study, using whole-cell voltage- clamp technique with Lucifer yellow in the recording solution, we demonstrate a positive correlation between the extent of dendritic outgrowth and the density of ACh currents, suggesting that these dendrites have ACh receptors. Our results indicate that neonatal rat nodose neurons are capable of extending dendrites and that extrinsic factors can induce or suppress their extension. In addition, the results suggest that these dendrites may act as principal post-synaptic structures for synapse formation that occurs in these cultures.

This article has been cited by other articles:

				V. Snitsarev, C. A. Whiteis, M. W. Chapleau, and F. M. Abboud Mechano- and chemosensitivity of rat nodose neurones - selective excitatory effects of prostacyclin J. Physiol., July 1, 2007; 582(1): 177 - 194. [Abstract] [Full Text] [PDF]

				V. Snitsarev, C. A. Whiteis, M. W. Chapleau, and F. M. Abboud Neuronal Prostacyclin Is an Autocrine Regulator of Arterial Baroreceptor Activity Hypertension, September 1, 2005; 46(3): 540 - 546. [Abstract] [Full Text] [PDF]

				S. Kraske, J. T. Cunningham, G. Hajduczok, M. W. Chapleau, F. M. Abboud, and R. E. Wachtel Mechanosensitive ion channels in putative aortic baroreceptor neurons Am J Physiol Heart Circ Physiol, October 1, 1998; 275(4): H1497 - H1501. [Abstract] [Full Text] [PDF]

				H. Kamiguchi and V. Lemmon A Neuronal Form of the Cell Adhesion Molecule L1 Contains a Tyrosine-Based Signal Required for Sorting to the Axonal Growth Cone J. Neurosci., May 15, 1998; 18(10): 3749 - 3756. [Abstract] [Full Text] [PDF]

				R. H. Friedel, H. Schnurch, J. Stubbusch, and Y.-A. Barde Identification of genes differentially expressed by nerve growth factor- and neurotrophin-3-dependent sensory neurons PNAS, November 11, 1997; 94(23): 12670 - 12675. [Abstract] [Full Text] [PDF]

				M. Rosenberg, B. Pie, and E. Cooper Developing Neonatal Rat Sympathetic and Sensory Neurons Differ in Their Regulation of 5-HT3 Receptor Expression J. Neurosci., September 1, 1997; 17(17): 6629 - 6638. [Abstract] [Full Text] [PDF]

				M. J. Sullivan, R. V. Sharma, R. E. Wachtel, M. W. Chapleau, L. J. Waite, R. C. Bhalla, and F. M. Abboud Non–Voltage-Gated Ca2+ Influx Through Mechanosensitive Ion Channels in Aortic Baroreceptor Neurons Circ. Res., June 19, 1997; 80(6): 861 - 867. [Abstract] [Full Text]

				M. W. Chapleau, J. T. Cunningham, M. J. Sullivan, R. E. Wachtel, and F. M. Abboud Structural Versus Functional Modulation of the Arterial Baroreflex Hypertension, August 1, 1995; 26(2): 341 - 347. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help