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Journal of Neuroscience, Vol 13, 1326-1336, Copyright © 1993 by Society for Neuroscience
Aggregation of sodium channels during development and maturation of the neuromuscular junction
MT Lupa, DM Krzemien, KL Schaller and JH Caldwell
Department of Cellular and Structural Biology, University of Colorado Health Science Center, Denver 80262.
The voltage-activated Na channel (NaCh) is an integral membrane protein
that is enriched at the neuromuscular end plate. Using loose-patch
voltage-clamp and immunofluorescence, we have found that the aggregation of
NaChs occurs late, during maturation of the neuromuscular junction. A
decline in expression of embryonic NaCh mRNA and increase in adult NaCh
mRNA precedes the onset of aggregation, and the appearance of functional
adult NaChs coincides with NaCh aggregation. We tested the possibility that
only the adult NaCh subtype could aggregate during development and found
that both the embryonic and adult isoforms become concentrated at the
synapse. The NaCh is the first postsynaptic membrane protein shown to
become clustered postnatally, and the mechanism producing this aggregation
appears to be different from the process producing aggregation of other
synaptic proteins.
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