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Journal of Neuroscience, Vol 13, 1551-1568, Copyright © 1993 by Society for Neuroscience
Immunocytochemical localization of a neuronal nicotinic receptor: the beta 2-subunit
JA Hill Jr, M Zoli, JP Bourgeois and JP Changeux
URA CNRS D1284, Neurobiologie Moleculaire, Institut Pasteur, Paris, France.
We have characterized in adult rat the tissue-specific expression of the
nicotinic ACh receptor (AChR) beta 2-subunit using antisera raised against
fusion protein constructs. Immunohistochemical localization revealed
immunoreactivity distributed throughout the neuraxis. Overall, beta 2-like
immunoreactivity (beta 2-LI) correlated well with in situ localization of
beta 2 transcript in neuronal cell bodies. Particularly strong labeling was
detected in the thalamus, and scattered other regions, whereas relatively
weak staining was observed in the hypothalamus and amygdala. At the
cellular level, beta 2-LI appeared to be exclusively neuronal and
concentrated predominantly in perikarya, although strongly positive
dendrites (cerebral cortical pyramidal neurons, cerebellar Purkinje cells)
and axon terminals (e.g., striatum) were detected. At the ultrastructural
level, beta 2-LI was membrane associated, with strong staining observed in
endoplasmic reticulum and cytoplasmic transport vesicles. beta 2-LI was
rarely detected at synapses. The widespread distribution of beta 2 suggests
it may serve as a common subunit in different AChR combinations in various
brain regions. Regulation of the expression of beta 2-subunit appears to be
relatively unrestrained, with an apparent excess of protein synthesized in
the cytoplasm relative to that which ultimately arrives at functional
targets in the plasma membrane.
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