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Journal of Neuroscience, Vol 13, 3006-3017, Copyright © 1993 by Society for Neuroscience
Molecular cloning of the murine JAK1 protein tyrosine kinase and its expression in the mouse central nervous system
X Yang, D Chung and CL Cepko
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Degenerate oligonucleotide primers were employed in PCRs to clone protein
tyrosine kinases that may play potential roles in the development of the
mammalian CNS. Using one PCR clone to screen a mouse eye cDNA library, a
full-length cDNA of a cytoplasmic tyrosine kinase, the homolog of human
JAK1, was obtained. The murine JAK1 kinase belongs to a new family of
cytoplasmic kinases that contain two tandem catalytic domains. Northern
analyses indicated that murine JAK1 mRNA is expressed in a variety of
tissues and cell lines. In the adult mouse eye, in situ hybridization and
immunohistochemistry showed that JAK1 mRNA and protein were expressed in
the retinal ganglion cell layer and the inner part of the inner nuclear
layer, presumably in amacrine cells. JAK1 protein was also detected in
horizontal cells and in the two synaptic layers of the adult retina. During
retinal development, JAK1 protein was first detected in retinal ganglion
cells and in their axons as early as embryonic day 14. Expression of JAK1
protein in amacrine cells and horizontal cells occurred only postnatally.
This pattern of expression was also observed in the chick retina,
suggesting an evolutionarily conserved function of JAK1 kinase in
vertebrate retinal development and/or function. Immunohistochemical
staining against JAK1 was detected in two areas of the adult mouse brain,
the olfactory bulb and a group of cells in the hypothalamus. Together,
these expression studies suggest a role for JAK1 kinase in the
differentiation or function of a subset of CNS neurons.
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