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Journal of Neuroscience, Vol 13, 3692-3704, Copyright © 1993 by Society for Neuroscience
Insulin-like receptor and insulin-like peptide are localized at neuromuscular junctions in Drosophila
M Gorczyca, C Augart and V Budnik
Department of Biology, University of Massachusetts, Amherst 01003.
Insulin and insulin-like growth factor (IGF) receptors are members of the
tyrosine kinase family of receptors, and are thought to play an important
role in the development and differentiation of neurons. Here we report the
presence of an insulin-like peptide and an insulin receptor (dInsR) at the
body wall neuromuscular junction of developing Drosophila larvae.
dInsR-like immunoreactivity was found in all body wall muscles at the motor
nerve branching regions, where it surrounded synaptic boutons. The identity
of this immunoreactivity as a dInsR was confirmed by two additional
schemes, in vivo binding of labeled insulin and immunolocalization of
phosphotyrosine. Both methods produced staining patterns markedly similar
to dInsR-like immunoreactivity. The presence of a dInsR in whole larvae was
also shown by receptor binding assays. This receptor was more specific for
insulin (> 25-fold) than for IGF II, and did not appear to bind IGF I.
Among the 30 muscle fibers per hemisegment, insulin-like immunoreactivity
was found only on one fiber, and was localized to a subset of
morphologically distinct synaptic boutons. Staining in the CNS was limited
to several cell bodies in the brain lobes and in a segmental pattern
throughout most of the abdominal ganglia, as well as in varicosities along
the neuropil areas of the ventral ganglion and brain lobes. Insulin-like
peptide and dInsR were first detected by early larval development, well
after neuromuscular transmission begins. The developmental significance of
an insulin-like peptide and its receptor at the neuromuscular junction is
discussed.
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