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Journal of Neuroscience, Vol 13, 3725-3735, Copyright © 1993 by Society for Neuroscience
Postmitotic expression of ankyrinR and beta R-spectrin in discrete neuronal populations of the rat brain
S Lambert and V Bennett
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
Isoforms of ankyrin (ankyrinR) are expressed in both the erythrocyte and
the brain. Four cDNAs representing regulatory domains of ankyrinR expressed
in the rat spleen and brain were cloned and sequenced. These different
cDNAs were found to result from tissue-specific alternative mRNA processing
of the ankyrinR regulatory domain. One of the isolated cDNAs was used to
develop an antibody to brain isoforms of ankyrinR, and this antibody was
used to study the localization of ankyrinR in the rat brain. The protein
was found to be widely expressed in neurons of the metencephalon but
limited to a discrete subset of neurons in the rat forebrain. In the
thalamus and areas of the basal ganglia, these neurons were grouped in
defined nuclei, whereas in the cortex, hippocampus, and caudate putamen
they appeared as isolated cells distributed randomly throughout these
structures. A similar study using an antibody raised against erythrocyte
spectrin (beta R) showed a comparable localization to that of ankyrinR.
Both proteins were expressed late in the developing rat brain, as part of
the maturation stage of neural development. These data suggest a specific
role for these erythrocyte structural proteins in the postmitotic
development of a subset of neurons in the rat brain.
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