 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 13, 3932-3943, Copyright © 1993 by Society for Neuroscience
Topographic patterns of brain activity in response to swim stress: assessment by 2-deoxyglucose uptake and expression of Fos-like immunoreactivity
GE Duncan, KB Johnson and GR Breese
Brain and Development Research Center, University of North Carolina at Chapel Hill 27599.
Alterations in brain activity patterns were assessed in response to swim
stress by immunocytochemical detection of Fos-like immunoreactivity
(Fos-LI) and high-resolution autoradiographic imaging of 14C-2-deoxyglucose
(2-DG) uptake. The stress paradigm investigated was a classic behavioral
screen for antidepressant drug activity, the forced swim test. One of the
most pronounced effects produced by swim stress was an increase in 2-DG
uptake and induction of Fos-LI in a restricted region of the lateral septal
nucleus. Specific "limbic" cortical regions, including the medial
prefrontal, ventrolateral orbital, and cingulate cortices, also exhibited
both increased 2-DG uptake and expression of Fos-LI in response to swim
stress. In the hypothalamic paraventricular nucleus of swim-stressed rats,
Fos-LI was induced but no change in 2-DG uptake was apparent. Since the
specific swim stress protocol used is a behavioral screen for
antidepressant drugs, the effects of imipramine on stress-induced
alterations in 2-DG uptake and induction of Fos-LI were examined. The
stress-induced increase in 2-DG uptake in the lateral septum was blocked by
treatment with imipramine, but treatment with imipramine had no effect on
induction of Fos-LI in the same region. Neither 2-DG uptake nor Fos-LI
expression was altered by imipramine treatment in the cortical regions
influenced by swim stress. Administration of imipramine alone under basal
conditions produced a robust induction of Fos-LI in the central nucleus of
the amygdala and in the dorsal lateral subdivision of the bed nucleus of
the stria terminalis. No effect of imipramine treatment on 2-DG uptake was
apparent in these latter regions. The results provide insights into
topographic patterns of brain activity associated with swim stress and
neuroanatomically selective actions of imipramine. The different and
complementary information obtained by assessment of Fos-LI and 2-DG uptake
illustrates the utility of applying both functional mapping approaches to
examine neuroanatomical correlates of behavioral states and drug treatment.
This article has been cited by other articles:
|
|
|
|
 
K. McFarland, S. B. Davidge, C. C. Lapish, and P. W. Kalivas
Limbic and Motor Circuitry Underlying Footshock-Induced Reinstatement of Cocaine-Seeking Behavior
J. Neurosci.,
February 18, 2004;
24(7):
1551 - 1560.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. E. Duncan, S. Miyamoto, and J. A. Lieberman
Chronic Administration of Haloperidol and Olanzapine Attenuates Ketamine-Induced Brain Metabolic Activation
J. Pharmacol. Exp. Ther.,
June 1, 2003;
305(3):
999 - 1005.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ritter, A. G. Watts, T. T. Dinh, G. Sanchez-Watts, and C. Pedrow
Immunotoxin Lesion of Hypothalamically Projecting Norepinephrine and Epinephrine Neurons Differentially Affects Circadian and Stressor-Stimulated Corticosterone Secretion
Endocrinology,
April 1, 2003;
144(4):
1357 - 1367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Roche, K. G. Commons, A. Peoples, and R. J. Valentino
Circuitry Underlying Regulation of the Serotonergic System by Swim Stress
J. Neurosci.,
February 1, 2003;
23(3):
970 - 977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. E. Duncan, S. Miyamoto, J. N. Leipzig, and J. A. Lieberman
Comparison of the Effects of Clozapine, Risperidone, and Olanzapine on Ketamine-Induced Alterations in Regional Brain Metabolism
J. Pharmacol. Exp. Ther.,
April 1, 2000;
293(1):
8 - 14.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. M. Rachman, J. R. Unnerstall, D. W. Pfaff, and R. S. Cohen
Estrogen alters behavior and forebrain c-fos expression in ovariectomized rats subjected to the forced swim test
PNAS,
November 10, 1998;
95(23):
13941 - 13946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. S. Hess, C. M. Gall, R. Granger, and G. Lynch
Differential Patterns of c-fos mRNA Expression in Amygdala During Successive Stages of Odor Discrimination Learning
Learn. Mem.,
September 1, 1997;
4(3):
262 - 283.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. G. Kirby and I. Lucki
Interaction Between the Forced Swimming Test and Fluoxetine Treatment on Extracellular 5-Hydroxytryptamine and 5-Hydroxyindoleacetic Acid in the Rat
J. Pharmacol. Exp. Ther.,
August 1, 1997;
282(2):
967 - 976.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Campeau, H. Akil, and S. J. Watson
Lesions of the Medial Geniculate Nuclei Specifically Block Corticosterone Release and Induction of c-fos mRNA in the Forebrain Associated with Audiogenic Stress in Rats
J. Neurosci.,
August 1, 1997;
17(15):
5979 - 5992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
