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Journal of Neuroscience, Vol 14, 5766-5777, Copyright © 1994 by Society for Neuroscience
The grasshopper median neuroblast is a multipotent progenitor cell that generates glia and neurons in distinct temporal phases
BG Condron and K Zinn
Division of Biology, California Institute of Technology, Pasadena 91125.
The midline of the insect CNS provides a good system for studying the
control of neuronal and glial cell fates, because it contains a small
number of distinct cell types that arise from a unique set of precursors.
In this report we analyze the development of the CNS midline in the
grasshopper embryo, focusing on the median neuroblast (MNB) cluster, which
contains the majority of midline neurons. We have directly traced cell
lineage in the MNB cluster by injecting the MNB with tracer dyes and
following the development of its progeny in whole embryo cultures. As least
three types of neuronal progeny are labeled with tracer dye in these
experiments. In addition, we find that all of the midline glia are also
derived from the MNB. Thus, this neuroblast is actually a multipotent
progenitor cell. We also examined the temporal and spatial patterns of
midline development by staining embryos with antibodies to neuronal and
glial markers and to the protein product of the engrailed gene, which is
transiently expressed by all MNB progeny. Our data show that neuronal and
glial progeny are generated from the MNB in distinct temporal phases. A
change in the orientation of the MNB's mitotic spindle correlates with the
transition between two of the phases of progeny production.
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