 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 5959-5972, Copyright © 1994 by Society for Neuroscience
Filopodia initiate choices made by sensory neuron growth cones at laminin/fibronectin borders in vitro
TM Gomez and PC Letourneau
University of Minnesota, Department of Cell Biology and Neuroanatomy, Minneapolis 55455.
Localized expression of environmental cues is thought to provide
directional information to migrating neuronal growth cones by enhancing or
suppressing axon outgrowth over limited regions. To investigate how such a
mechanism may function in vivo, we observed growth cones of embryonic chick
dorsal root ganglion neurons at a substratum border between the
extracellular matrix components laminin and fibronectin in vitro using
time-lapse phase-contrast and interference reflection microscopy. We found
that patterns of laminin and fibronectin could locally promote or suppress
the direction of growth cone migration. While migrating on either laminin
or fibronectin, at least 79% of growth cones changed their rate and/or
direction of outgrowth upon contact with the alternative substratum, in a
manner suggesting that growth cones were selecting one substratum over the
other. Complex changes in growth cone behavior were initiated by filopodial
contact with the alternate substratum, suggesting that filopodia were
providing intracellular signals to the growth cone. Using interference
reflection microscopy, we have found that selection of a substratum is
independent of the degree of close contact to the substratum. We conclude
that spatially localized ECM components can direct axon outgrowth by
mechanisms based on intracellular signaling through growth cone filopodia.
This article has been cited by other articles:
|
|
|
|
 
S. Gehler, A. E. Shaw, P. D. Sarmiere, J. R. Bamburg, and P. C. Letourneau
Brain-Derived Neurotrophic Factor Regulation of Retinal Growth Cone Filopodial Dynamics Is Mediated through Actin Depolymerizing Factor/Cofilin
J. Neurosci.,
November 24, 2004;
24(47):
10741 - 10749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Hynds, M. L. Spencer, D. A. Andres, and D. M. Snow
Rit promotes MEK-independent neurite branching in human neuroblastoma cells
J. Cell Sci.,
May 15, 2003;
116(10):
1925 - 1935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Kim, P. Kolodziej, and A. Chiba
Growth Cone Pathfinding and Filopodial Dynamics Are Mediated Separately by Cdc42 Activation
J. Neurosci.,
March 1, 2002;
22(5):
1794 - 1806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Steketee, K. Balazovich, and K. W. Tosney
Filopodial Initiation and a Novel Filament-organizing Center, the Focal Ring
Mol. Biol. Cell,
August 1, 2001;
12(8):
2378 - 2395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Gomez, E. Robles, M.-m. Poo, and N. C. Spitzer
Filopodial Calcium Transients Promote Substrate-Dependent Growth Cone Turning
Science,
March 9, 2001;
291(5510):
1983 - 1987.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Polinsky, K. Balazovich, and K. W. Tosney
Identification of an Invariant Response: Stable Contact with Schwann Cells Induces Veil Extension in Sensory Growth Cones
J. Neurosci.,
February 1, 2000;
20(3):
1044 - 1055.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Condic, D. M. Snow, and P. C. Letourneau
Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression
J. Neurosci.,
November 15, 1999;
19(22):
10036 - 10043.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Meiners, M. L. T. Mercado, M. S. A. Nur-e-Kamal, and H. M. Geller
Tenascin-C Contains Domains That Independently Regulate Neurite Outgrowth and Neurite Guidance
J. Neurosci.,
October 1, 1999;
19(19):
8443 - 8453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Isbister and T. P. O'Connor
Filopodial Adhesion Does Not Predict Growth Cone Steering Events In Vivo
J. Neurosci.,
April 1, 1999;
19(7):
2589 - 2600.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gallo and P. C. Letourneau
Localized Sources of Neurotrophins Initiate Axon Collateral Sprouting
J. Neurosci.,
July 15, 1998;
18(14):
5403 - 5414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. H. J. Aarts, L. H. Schrama, W. J. Hage, J. L. Bos, W. H. Gispen, and P. Schotman
B-50/GAP-43-induced Formation of Filopodia Depends on Rho-GTPase
Mol. Biol. Cell,
June 1, 1998;
9(6):
1279 - 1292.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Gallo, F. B. Lefcort, and P. C. Letourneau
The trkA Receptor Mediates Growth Cone Turning toward a Localized Source of Nerve Growth Factor
J. Neurosci.,
July 15, 1997;
17(14):
5445 - 5454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Mason and L.-C. Wang
Growth Cone Form Is Behavior-Specific and, Consequently, Position-Specific along the Retinal Axon Pathway
J. Neurosci.,
February 1, 1997;
17(3):
1086 - 1100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L Garcia-Alonso, R. Fetter, and C. Goodman
Genetic analysis of Laminin A in Drosophila: extracellular matrix containing laminin A is required for ocellar axon pathfinding
Development,
January 9, 1996;
122(9):
2611 - 2621.
[Abstract]
[PDF]
|
|
|
|
|
