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Journal of Neuroscience, Vol 14, 6370-6383, Copyright © 1994 by Society for Neuroscience
DLX-2, MASH-1, and MAP-2 expression and bromodeoxyuridine incorporation define molecularly distinct cell populations in the embryonic mouse forebrain
MH Porteus, A Bulfone, JK Liu, L Puelles, LC Lo and JL Rubenstein
Nina Ireland Laboratory of Developmental Biology, Department of Psychiatry, University of California, San Francisco 94143-0984.
Recently, the Dlx family of homeobox genes have been identified as
candidates for regulating patterning and differentiation of the forebrain.
We have made a polyclonal antiserum to the protein product of the Dlx-2
gene. Using this antiserum, we have characterized the spatial and temporal
pattern of DLX-2 protein expression during murine development and in the
adult mouse brain. These studies demonstrate that, like the mRNA from the
Dlx-2 gene, DLX-2 protein is expressed in mouse embryonic forebrain, limbs,
tail, genital tubercle, and branchial arches. Within the embryonic
forebrain, DLX-2 protein is expressed within specific transverse and
longitudinal domains. Analysis of expression within the wall of the
forebrain shows that DLX-2 is expressed in proliferative regions including
the ventricular and subventricular zones. DLX-2 is expressed in the same
cells as MASH-1, a marker of relatively undifferentiated cells, but in a
reciprocal fashion to MAP-2, a marker of terminal neuronal differentiation.
A number of DLX-2-expressing cells, but not all, can be labeled with
bromodeoxyuridine (BrdU). Using the patterns of DLX-2, MASH-1, MAP-2
expression, and bromodeoxyuridine incorporation, we identify four
molecularly distinct populations of cells that may correspond to different
stages of neuronal differentiation in the mouse basal forebrain, in which
DLX-2 is expressed at the transition from proliferation to terminal
differentiation.
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