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Journal of Neuroscience, Vol 14, 6423-6434, Copyright © 1994 by Society for Neuroscience
Expression of neuropeptide Y and neuropeptide Y (Y1) receptor mRNA in rat spinal cord and dorsal root ganglia following peripheral tissue inflammation
RR Ji, X Zhang, Z Wiesenfeld-Hallin and T Hokfelt
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
By using in situ hybridization histochemistry and immunohistochemistry,
neuropeptide Y (NPY) and NPY (Y1) receptor mRNA as well as NPY-like
immunoreactivity were examined in the lumbar spinal cord (L4-L5) and in
dorsal root ganglia (DRG, L5) in rats injected with complete Freund's
adjuvant (CFA) into the hindpaw. A rapid and marked increase in NPY mRNA
expression was observed in ipsilateral dorsal horn neurons 6 hr after
inoculation as compared to the contralateral side. This was mainly found in
the medial part of spinal lamina II. The peak level (88% increase) was
reached at 3 d. In adjacent sections of the spinal cord, 96% and 33%
increases were found in the number of dynorphin and enkephalin
mRNA-positive neurons, respectively. Unilateral inflammation also induced a
moderate increase in NPY-like immunoreactivity and the number of
NPY-immunoreactive neurons in the medial part of the ipsilateral spinal
dorsal horn. In addition, a marked elevation in the expression of
c-Fos-like protein was observed in ipsilateral spinal neurons in laminae I,
II, and V. However, no NPY mRNA-positive or NPY- immunoreactive neurons
were found in the ipsilateral and contralateral DRGs in rats receiving CFA
injection. Furthermore, a marked upregulation of NPY (Y1) receptor mRNA
expression was detected in the ipsilateral spinal dorsal horn 1 d and 3 d
after inoculation. These Y1 receptor mRNA-positive cells were mainly
distributed in the medial laminae II and III. Numerous Y1 mRNA-positive,
small neuron profiles were found bilaterally in the DRGs in CFA-treated
rats. CFA evoked a 34% increase in the number of Y1 mRNA-positive neurons
in ipsilateral DRGs as compared to contralateral DRGs. The distinct
upregulation of NPY and NPY (Y1) receptor in response to peripheral
inflammation suggests an involvement of NPY in the response to inflammation
and in nociception.
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