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Journal of Neuroscience, Vol 14, 6667-6686, Copyright © 1994 by Society for Neuroscience
Cellular and molecular characterization of Ca2+ currents in acutely isolated, adult rat neostriatal neurons
J Bargas, A Howe, J Eberwine, Y Cao and DJ Surmeier
Instituto de Fisologia Celular, Universidad Nacional Autonoma de Mexico, Mexico.
Ca2+ currents in acutely isolated, adult rat neostriatal neurons were
studied with whole-cell voltage-clamp techniques. In the vast majority of
neurons (approximately 90%, n > 250), currents were exclusively of the
high-voltage-activated (HVA) type. HVA currents activated near -40 mV and
reached their maximum amplitude near 0 mV. Quasi-steady-state inactivation
curves in many neurons were well fitted only with a sum of Boltzmann
functions, suggesting that the HVA currents were heterogeneous. Although
the block of whole-cell current by Cd2+ was well fitted with a single
isotherm having an IC50 of near 1 microM, experiments with organic channel
antagonists suggested that at least four types of HVA channels were
expressed by most cells. On average, the L-channel antagonist nifedipine
(5-10 microM) blocked 31 +/- 10% of the whole-cell current (n = 20), the
N-channel antagonist omega- conotoxin GVIA (omega-CgTx) (2-5 microM)
blocked 27 +/- 11% (n = 20), and the P-channel antagonist omega-agatoxin
IVA (100-500 nM) blocked 21 +/- 10% (n = 18). In many neurons, the block by
omega-CgTx was partially or completely reversible. In cells tested with a
combination of these antagonists, 34 +/- 17% of the peak Ca2+ current
remained unblocked (n = 13). Single-cell expression profiling of
medium-sized neurons revealed the presence of rbA and rbB Ca2+ channel
alpha 1 subunit mRNAs but low or undetectable levels of rbC mRNA (n = 12).
These findings suggest that although adult neostriatal projection neurons
do not express significant levels of LVA Ca2+ current, they do express a
pharmacologically and structurally heterogeneous population of HVA
currents.
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