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Journal of Neuroscience, Vol 14, 7272-7283, Copyright © 1994 by Society for Neuroscience
A Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the "HRP" carbohydrate epitope
CJ Desai, E Popova and K Zinn
Division of Biology, California Institute of Technology, Pasadena 91125.
Recent studies have defined several cell surface glycoproteins expressed in
the developing nervous system of insect embryos that may be involved in
axon outgrowth and guidance processes. These glycoproteins include the
fasciclins and a group of receptor-linked protein tyrosine phosphatases
(R-PTPs). In embryos, the fasciclins are localized to axonal subsets, while
the R-PTPs appear to be expressed on most or all CNS axons. To identify
other neuronal cell surface glycoproteins in the Drosophila embryo, we have
taken a biochemical approach. This is based on the observation that
antisera against horseradish peroxidase (HRP) recognize a carbohydrate
epitope that is selectively expressed in the insect nervous system. A large
number of neuronal glycoproteins (denoted "HRP proteins") apparently bear
the HRP carbohydrate epitope. We have used polyclonal anti-HRP antibodies
to purify these proteins from Drosophila embryos, and have obtained protein
sequences from seven HRP protein bands. These data define three major HRP
proteins as neurotactin, fasciclin I, and an R-PTP, DPTP69D. Western
blotting data suggest that fasciclin II, neuroglian, DPTP10D, and DPTP99A
are also HRP proteins. We show that DPTP69D, like the previously
characterized R-PTPs, is localized to CNS axons in the embryo. In third
instar larvae, DPTP69D expression is restricted to subsets of neuronal
processes in the brain, ventral nerve cord, and eye disk. In the optic
lobes, DPTP69D is localized to the neuropils of the lamina and medulla, and
to an array of parallel thick bundles that may be the transmedullary fibers
of the developing lobula complex.
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