Journal of Neuroscience, Vol 14, 7469-7477, Copyright © 1994 by Society for Neuroscience
Age-related decrease of GABAA receptor subunits and glutamic acid decarboxylase in the rat inferior colliculus
A Gutierrez, ZU Khan, SJ Morris and AL De Blas
Division of Molecular Biology and Biochemistry, School of Biological Science, University of Missouri-Kansas City 64110-2499.
A selective age-related decrease in both the protein and mRNA levels of the
most abundant GABAA receptor subunits has been revealed in the rat inferior
colliculus. The number (not affinity) of the native and fully assembled
GABAA receptors assayed by 3H-muscimol binding was also decreased (35-49%).
The decrease in GABA receptors was accompanied by a decrease in the protein
and mRNA of the GABA-synthesizing enzyme glutamic acid decarboxylase. No
other region of the rat brain showed such large age-related changes in
these GABAergic synaptic molecules. Specific antibodies and riboprobes in
conjunction with a computerized image analysis system were used to quantify
immunocytochemistry and in situ hybridization. In old Sprague-Dawley rats,
the combination of beta 2 and beta 3 peptide subunits was reduced 55%,
while the beta 2 and beta 3 mRNAs were decreased 31% and 22%, respectively.
The gamma 2S and gamma 2L subunit proteins decreased 43% and 21%,
respectively, while the gamma 2 mRNA, including both short and long forms,
was reduced 61%. The alpha 1 subunit protein was decreased 28%, whereas the
alpha 1 mRNA decreased 40%. The glutamic acid decarboxylase protein was
reduced 62% while GAD65 mRNA decreased 42%. Similar age-related changes
were also observed in the inferior colliculus of Fischer-344 rats. In
contrast, no changes were observed in the level of expression of some glial
and/or neuronal proteins such as S-100, glial fibrillary acidic protein,
and 160 KDa neurofilament protein in the inferior colliculus.(ABSTRACT
TRUNCATED AT 250 WORDS)
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