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Journal of Neuroscience, Vol 14, 499-510, Copyright © 1994 by Society for Neuroscience
GAP-43 transgenic mice: dispersed genomic sequences confer a GAP-43- like expression pattern during development and regeneration
J Vanselow, E Grabczyk, J Ping, M Baetscher, S Teng and MC Fishman
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
Using transgenic mice, we have examined the expression pattern conferred by
regions of genomic GAP-43 coupled to beta-galactosidase. We demonstrate
that gene constructions that include the GAP-43 5'- flanking region along
with sufficient sequences of the first intron drive beta-galactosidase
(lacZ) expression to mimic in many regards the complex spatial and temporal
pattern of endogenous GAP-43 expression. Transgene expression reaches peak
levels during development, and persists at high levels in particular adult
brain regions, such as the hippocampus and olfactory bulb. The inclusion of
a stretch of the first intron in the construction is necessary to prevent
expression outside of the nervous system, indicating that some of the cell
specificity of GAP-43 expression is due to suppression of expression in
inappropriate tissues. Injury caused by sciatic nerve crush causes
reexpression of the transgene in adult sensory and motor neurons. This
genomic region of GAP-43, therefore, includes elements responsive to
neuronal growth signals that regulate both development and regeneration.
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