 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 857-870, Copyright © 1994 by Society for Neuroscience
Microtubule-associated protein 1b (MAP1b) is concentrated in the distal region of growing axons
MM Black, T Slaughter and I Fischer
Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Microtubule-associated protein 1b (MAP1b) is expressed at especially high
levels in neurons actively extending axons, and although it appears to be
required for axon growth, the nature of its role is unknown. We reasoned
that a detailed description of the localization of MAP1b in growing axons
would help define how MAP1b participates in axon growth. Therefore, we have
stained cultured sympathetic neurons with various antibodies against MAP1b,
and then used digital image processing and analysis procedures to quantify
MAP1b distribution, phosphorylation and association with microtubules (MTs)
in actively elongating axons. MAP1b is present on MTs all along the axon.
Quantitative analyses showed that MAP1b has a nonuniform distribution along
growing axons. It is present at relatively low and constant levels along
the axon shaft until approximately 130 microns from the axon tip, where the
amount of MAP1b begins to increase sharply and reaches a peak close to the
growth cone. The peak amount of MAP1b in the distal axon is an order of
magnitude greater than the average amount in the axon shaft. The enrichment
of MAP1b in the distal axon was observed for total MAP1b and assembled
MAP1b, and was even more pronounced for phosphorylated MAP1b. This
distribution pattern remains after correcting the relative amount of MAP1b
along the axon for variations in axonal volume. Thus, the concentration of
MAP1b in the distal axon exceeds by severalfold that in the rest of the
axon. The amount of assembled MAP1b relative to the amount of MT polymer
also varies along the axon, and is greatest distally near the growth cone.
This pattern of MAP1b localization in axons focuses attention on the distal
axon and growth cone as the principal sites of MAP1b function in axon
growth. We discuss the possibility that MAP1b regulates MT dynamics in the
distal axon so that it is properly coordinated with growth cone events
involved in axon extension.
This article has been cited by other articles:
|
|
|
|
 
M. P. Koester, O. Muller, and G. E. Pollerberg
Adenomatous Polyposis Coli Is Differentially Distributed in Growth Cones and Modulates Their Steering
J. Neurosci.,
November 14, 2007;
27(46):
12590 - 12600.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Galtrey, R. A. Asher, F. Nothias, and J. W. Fawcett
Promoting plasticity in the spinal cord with chondroitinase improves functional recovery after peripheral nerve repair
Brain,
April 1, 2007;
130(4):
926 - 939.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-E. Allain, P. Meyrand, and P. Branchereau
Ontogenic Changes of the Spinal GABAergic Cell Population Are Controlled by the Serotonin (5-HT) System: Implication of 5-HT1 Receptor Family
J. Neurosci.,
September 21, 2005;
25(38):
8714 - 8724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Laurino, X. X. Wang, B. A. de la Houssaye, L. Sosa, S. Dupraz, A. Caceres, K. H. Pfenninger, and S. Quiroga
PI3K activation by IGF-1 is essential for the regulation of membrane expansion at the nerve growth cone
J. Cell Sci.,
August 15, 2005;
118(16):
3653 - 3662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Paganoni and A. Ferreira
Neurite extension in central neurons: a novel role for the receptor tyrosine kinases Ror1 and Ror2
J. Cell Sci.,
January 15, 2005;
118(2):
433 - 446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Bouquet, S. Soares, Y. von Boxberg, M. Ravaille-Veron, F. Propst, and F. Nothias
Microtubule-Associated Protein 1B Controls Directionality of Growth Cone Migration and Axonal Branching in Regeneration of Adult Dorsal Root Ganglia Neurons
J. Neurosci.,
August 11, 2004;
24(32):
7204 - 7213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. B. Mantych and A. Ferreira
Agrin Differentially Regulates the Rates of Axonal and Dendritic Elongation in Cultured Hippocampal Neurons
J. Neurosci.,
September 1, 2001;
21(17):
6802 - 6809.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Gonzalez-Billault, J. Avila, and A. Caceres
Evidence for the Role of MAP1B in Axon Formation
Mol. Biol. Cell,
July 1, 2001;
12(7):
2087 - 2098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Meixner, S. Haverkamp, H. Wassle, S. Fuhrer, J. Thalhammer, N. Kropf, R. E. Bittner, H. Lassmann, G. Wiche, and F. Propst
Map1b Is Required for Axon Guidance and Is Involved in the Development of the Central and Peripheral Nervous System
J. Cell Biol.,
December 11, 2000;
151(6):
1169 - 1178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Takei, J. Teng, A. Harada, and N. Hirokawa
Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes
J. Cell Biol.,
September 4, 2000;
150(5):
989 - 1000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ma, B. T. Himes, T. B. Shea, and I. Fischer
Axonal Transport of Microtubule-Associated Protein 1B (MAP1B) in the Sciatic Nerve of Adult Rat: Distinct Transport Rates of Different Isoforms
J. Neurosci.,
March 15, 2000;
20(6):
2112 - 2120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Goold, R Owen, and P. Gordon-Weeks
Glycogen synthase kinase 3beta phosphorylation of microtubule-associated protein 1B regulates the stability of microtubules in growth cones
J. Cell Sci.,
January 10, 1999;
112(19):
3373 - 3384.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. Paglini, G. Pigino, P. Kunda, G. Morfini, R. Maccioni, S. Quiroga, A. Ferreira, and A. Caceres
Evidence for the Participation of the Neuron-Specific CDK5 Activator P35 during Laminin-Enhanced Axonal Growth
J. Neurosci.,
December 1, 1998;
18(23):
9858 - 9869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Togel, G. Wiche, and F. Propst
Novel Features of the Light Chain of Microtubule-associated Protein MAP1B: Microtubule Stabilization, Self Interaction, Actin Filament Binding, and Regulation by the Heavy Chain
J. Cell Biol.,
November 2, 1998;
143(3):
695 - 707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Tint, T. Slaughter, I. Fischer, and M. M. Black
Acute Inactivation of Tau Has No Effect on Dynamics of Microtubules in Growing Axons of Cultured Sympathetic Neurons
J. Neurosci.,
November 1, 1998;
18(21):
8660 - 8673.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Brown
Contiguous phosphorylated and non-phosphorylated domains along axonal neurofilaments
J. Cell Sci.,
January 2, 1998;
111(4):
455 - 467.
[Abstract]
[PDF]
|
|
|
|
|
|
|
T. Slaughter, J. Wang, and M. M. Black
Microtubule Transport from the Cell Body into the Axons of Growing Neurons
J. Neurosci.,
August 1, 1997;
17(15):
5807 - 5819.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mascotti, A. Caceres, K. H. Pfenninger, and S. Quiroga
Expression and Distribution of IGF-1 Receptors Containing a beta -Subunit Variant (beta gc) in Developing Neurons
J. Neurosci.,
February 15, 1997;
17(4):
1447 - 1459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G Pigino, G Paglini, L Ulloa, J Avila, and A Caceres
Analysis of the expression, distribution and function of cyclin dependent kinase 5 (cdk5) in developing cerebellar macroneurons
J. Cell Sci.,
January 1, 1997;
110(2):
257 - 270.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Wang, W. Yu, P. W. Baas, and M. M. Black
Microtubule Assembly in Growing Dendrites
J. Neurosci.,
October 1, 1996;
16(19):
6065 - 6078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Mandell and G. A. Banker
A Spatial Gradient of Tau Protein Phosphorylation in Nascent Axons
J. Neurosci.,
September 15, 1996;
16(18):
5727 - 5740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Liu and I. Fischer
Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene
J. Neurosci.,
August 15, 1996;
16(16):
5026 - 5036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Black, T. Slaughter, S. Moshiach, M. Obrocka, and I. Fischer
Tau Is Enriched on Dynamic Microtubules in the Distal Region of Growing Axons
J. Neurosci.,
June 1, 1996;
16(11):
3601 - 3619.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Zachow and D Bentley
Blackjack, a novel protein associated with microtubules in embryonic neurons
J. Cell Sci.,
January 6, 1996;
109(6):
1497 - 1507.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A Ferreira and K. Kosik
Accelerated neuronal differentiation induced by p53 suppression
J. Cell Sci.,
January 6, 1996;
109(6):
1509 - 1516.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. DiTella, F Feiguin, N Carri, K. Kosik, and A Caceres
MAP-1B/TAU functional redundancy during laminin-enhanced axonal growth
J. Cell Sci.,
January 2, 1996;
109(2):
467 - 477.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Bonnet, D. Boucher, S. Lazereg, B. Pedrotti, K. Islam, P. Denoulet, and J. C. Larcher
Differential Binding Regulation of Microtubule-associated Proteins MAP1A, MAP1B, and MAP2 by Tubulin Polyglutamylation
J. Biol. Chem.,
April 13, 2001;
276(16):
12839 - 12848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
