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Journal of Neuroscience, Vol 14, 949-972, Copyright © 1994 by Society for Neuroscience
Differential expression of Shaw-related K+ channels in the rat central nervous system
M Weiser, E Vega-Saenz de Miera, C Kentros, H Moreno, L Franzen, D Hillman, H Baker and B Rudy
Department of Physiology and Biophysics, New York University Medical Center, New York 10016.
The family of mammalian genes related to the Drosophila Shaker gene,
consisting of four subfamilies, is thought to encode subunits of tetrameric
voltage-gated K+ channels. There is compelling evidence that subunits of
the same subfamily, but not of different subfamilies, form heteromultimeric
channels in vitro, and thus, each gene subfamily is postulated to encode
components of an independent channel system. In order to identify cells
with native channels containing subunits of one of these subfamilies
(Shaw-related or ShIII), the cellular distribution of ShIII transcripts was
examined by Northern blot analysis and in situ hybridization. Three of four
ShIII genes (KV3.1, KV3.2, and KV3.3) are expressed mainly in the CNS.
KV3.4 transcripts are also present in the CNS but are more abundant in
skeletal muscle. In situ hybridization studies in the CNS reveal discrete
and specific neuronal populations that prominently express ShIII mRNAs,
both in projecting and in local circuit neurons. In the cerebral cortex,
hippocampus, and caudate- putamen, subsets of neurons can be distinguished
by the expression of specific ShIII mRNAs. Each ShIII gene exhibits a
unique pattern of expression; however, many neuronal populations expressing
KV3.1 transcripts also express KV3.3 mRNAs. Furthermore, KV3.4 transcripts
are present, albeit at lower levels, in several of the neuronal populations
that also express KV3.1 and/or KV3.3 mRNAs, revealing a high potential for
heteromultimer formation between the products of three of the four genes.
Expression of ShIII cRNAs in Xenopus oocytes was used to explore the
functional consequences of heteromultimer formation between ShIII subunits.
Small amounts of KV3.4 cRNA, which expresses small, fast-inactivating
currents when injected alone, produced fast-inactivating currents that are
severalfold larger when coinjected with an excess of KV3.1 or KV3.3 cRNA.
This amplification is due to both an increase in single-channel conductance
in the heteromultimeric channels and the observation that less than four,
perhaps even a single KV3.4 subunit is sufficient to impart fast-
inactivating properties to the channel. The oocyte experiments indicate
that the apparently limited, low-level expression of KV3.4 in the CNS is
potentially significant. The anatomical studies suggest that heteromultimer
formation between ShIII proteins might be a common feature in the CNS.
Moreover, the possibility that the subunit composition of heteromultimers
varies in different neurons should be considered, since the ratios of
overlapping signals change from one neuronal population to another. In
order to proceed with functional analysis of native ShIII channels, it is
important to known which subunit compositions might occur in vivo. The
studies presented here provide important clues for the identification of
native homo- and heteromultimeric ShIII channels in neurons.
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R. H. Joho, C. S. Ho, and G. A. Marks
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R. Hernandez-Pineda, A. Chow, Y. Amarillo, H. Moreno, M. Saganich, E. V.-S. de Miera, A. Hernandez-Cruz, and B. Rudy
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G. Baranauskas, T. Tkatch, and D. J. Surmeier
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S. Takeuchi and M. Ando
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P. O. Kanold and P. B. Manis
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J. T. Blaine and A. B. Ribera
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S.-q. J. Liu and L. K. Kaczmarek
Depolarization Selectively Increases the Expression of the Kv3.1 Potassium Channel in Developing Inferior Colliculus Neurons
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[Abstract]
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M. Martina, J. H. Schultz, H. Ehmke, H. Monyer, and P. Jonas
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K. P. Giese, J. F. Storm, D. Reuter, N. B. Fedorov, L.-R. Shao, T. Leicher, O. Pongs, and A. J. Silva
Reduced K+ Channel Inactivation, Spike Broadening, and After-Hyperpolarization in Kvbeta 1.1-Deficient Mice with Impaired Learning
Learn. Mem.,
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257 - 273.
[Abstract]
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R. M. Sanchez, A. Surkis, and C. S. Leonard
Voltage-Clamp Analysis and Computer Simulation of a Novel Cesium-Resistant A-Current in Guinea Pig Laterodorsal Tegmental Neurons
J Neurophysiol,
June 1, 1998;
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3111 - 3126.
[Abstract]
[Full Text]
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L. A. Gabel and E. S. Nisenbaum
Biophysical Characterization and Functional Consequences of a Slowly Inactivating Potassium Current in Neostriatal Neurons
J Neurophysiol,
April 1, 1998;
79(4):
1989 - 2002.
[Abstract]
[Full Text]
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P. Serodio and B. Rudy
Differential Expression of Kv4 K+ Channel Subunits Mediating Subthreshold Transient K+ (A-Type) Currents in Rat Brain
J Neurophysiol,
February 1, 1998;
79(2):
1081 - 1091.
[Abstract]
[Full Text]
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A. P. Southan and B. Robertson
Patch-Clamp Recordings from Cerebellar Basket Cell Bodies and Their Presynaptic Terminals Reveal an Asymmetric Distribution of Voltage-Gated Potassium Channels
J. Neurosci.,
February 1, 1998;
18(3):
948 - 955.
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A. Castellano, M. D. Chiara, B. Mellstrom, A. Molina, F. Monje, J. R. Naranjo, and J. Lopez-Barneo
Identification and Functional Characterization of a K+ Channel alpha -Subunit with Regulatory Properties Specific to Brain
J. Neurosci.,
June 15, 1997;
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J. L. Massengill, M. A. Smith, D. I. Son, and D. K. O'Dowd
Differential Expression of K4-AP Currents and Kv3.1 Potassium Channel Transcripts in Cortical Neurons that Develop Distinct Firing Phenotypes
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May 1, 1997;
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