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Journal of Neuroscience, Vol 14, 1943-1952, Copyright © 1994 by Society for Neuroscience
Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor
PH King, TD Levine, RT Fremeau Jr and JD Keene
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710.
Mammalian cDNAs encoding a rat (Rel-N1) and a human (Hel-N1) neuronal
RNA-binding protein have been cloned and characterized with respect to
tissue specificity, neuroanatomical localization, and RNA binding
specificity. Both proteins are highly similar to the product of the
Drosophila elav gene, which is expressed in all neurons of the fly and is
required for development of the nervous system. However, in situ
hybridization of rat tissues demonstrated more restricted expression of
Rel-N1 mRNA within a subset of neurons of the hippocampus, cortex, and
other regions of the gray matter, but not in glial cells or white matter.
In vitro RNA binding experiments demonstrated that Hel-N1 can bind to the
3' untranslated region (3' UTR) of Id mRNA, a transcript that encodes a
helix-loop-helix transcriptional repressor that is abundantly expressed in
undifferentiated neural precursors. Sequences characterized for Hel-N1
binding were also abundantly present in the 3' UTR of the Drosophila
extramacrochaetae mRNA, which encodes an Id homolog. Thus, we have
identified a potential link between a neuronal 3' UTR RNA-binding protein
and regulatory transcription factors involved in neural development. These
findings are interpreted in light of recent studies in which mRNA 3' UTRs
were found to be important for the regulation of cell growth and
differentiation.
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