 |
Next Article 
Journal of Neuroscience, Vol 14, 2455-2463, Copyright © 1994 by Society for Neuroscience
Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements
GW Hoyle, EH Mercer, RD Palmiter and RL Brinster
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
The promoter region of the human dopamine beta-hydroxylase (DBH) gene was
analyzed in transgenic mice to identify DNA sequences responsible for the
tissue- and cell-specific expression of the gene. Transgenic mice were
generated that carried the Escherichia coli lacZ gene under control of DBH
promoter fragments between 0.6 and 5.8 kilobases (kb) in length. Sequences
required for expression in adult and fetal noradrenergic neurons were
located between 0.6 and 1.1 kb 5' to the DBH transcriptional start site.
Sequences in this region and farther upstream also directed expression to
dopaminergic and noncatecholaminergic brain neurons that was repressed by
negative elements elsewhere in the gene. The results indicate that the
neuron- specific expression of the DBH gene is mediated by positive
regulatory elements but that negative elements are required to restrict
expression to the proper subset of neurons.
This article has been cited by other articles:
|
|
|
|
 
M. Stanke, C. V. Duong, M. Pape, M. Geissen, G. Burbach, T. Deller, H. Gascan, R. Parlato, G. Schutz, and H. Rohrer
Target-dependent specification of the neurotransmitter phenotype: cholinergic differentiation of sympathetic neurons is mediated in vivo by gp130 signaling
Development,
January 1, 2006;
133(1):
141 - 150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Kim, D.-Y. Hwang, J.-J. Park, and K.-S. Kim
A Proximal Promoter Domain Containing a Homeodomain-Binding Core Motif Interacts with Multiple Transcription Factors, Including HoxA5 and Phox2 Proteins, and Critically Regulates Cell Type-Specific Transcription of the Human Norepinephrine Transporter Gene
J. Neurosci.,
April 1, 2002;
22(7):
2579 - 2589.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Steiner, J. G. Hohmann, A. Holmes, C. C. Wrenn, G. Cadd, A. Juréus, D. K. Clifton, M. Luo, M. Gutshall, S. Y. Ma, et al.
Galanin transgenic mice display cognitive and neurochemical deficits characteristic of Alzheimer's disease
PNAS,
March 16, 2001;
(2001)
61445598.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. A. Rivkees, M. Chen, J. Kulkarni, J. Browne, and Z. Zhao
Characterization of the Murine A1 Adenosine Receptor Promoter, Potent Regulation by GATA-4 and Nkx2.5
J. Biol. Chem.,
May 14, 1999;
274(20):
14204 - 14209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Kim, H.-S. Kim, J. F. Cubells, and K.-S. Kim
A Previously Undescribed Intron and Extensive 5' Upstream Sequence, but Not Phox2a-mediated Transactivation, Are Necessary for High Level Cell Type-specific Expression of the Human Norepinephrine Transporter Gene
J. Biol. Chem.,
March 5, 1999;
274(10):
6507 - 6518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-S. Kim, H. Seo, C. Yang, J.-F. Brunet, and K.-S. Kim
Noradrenergic-Specific Transcription of the Dopamine beta -Hydroxylase Gene Requires Synergy of Multiple Cis-Acting Elements Including at Least Two Phox2a-Binding Sites
J. Neurosci.,
October 15, 1998;
18(20):
8247 - 8260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. F. Landry, T. M. Pribyl, J. A. Ellison, M. I. Givogri, K. Kampf, C. W. Campagnoni, and A. T. Campagnoni
Embryonic Expression of the Myelin Basic Protein Gene: Identification of a Promoter Region That Targets Transgene Expression to Pioneer Neurons
J. Neurosci.,
September 15, 1998;
18(18):
7315 - 7327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Fawcett, S. X. Bamji, C. G. Causing, R. Aloyz, A. R. Ase, T. A. Reader, J. H. McLean, and F. D. Miller
Functional Evidence that BDNF Is an Anterograde Neuronal Trophic Factor in the CNS
J. Neurosci.,
April 15, 1998;
18(8):
2808 - 2821.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Strayer, S. H. Guttentag, and P. L. Ballard
Targeting Type II and Clara Cells for Adenovirus-mediated Gene Transfer Using the Surfactant Protein B Promoter
Am. J. Respir. Cell Mol. Biol.,
January 1, 1998;
18(1):
1 - 9.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Sekerkova, Z. Katarova, F. Joo, J. R. Wolff, S. Prodan, and G. Szabo
Visualization of {beta}-Galactosidase by Enzyme and Immunohistochemistry in the Olfactory Bulb of Transgenic Mice Carrying the LacZ Transgene
J. Histochem. Cytochem.,
August 1, 1997;
45(8):
1147 - 1156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schoch, G. Cibelli, and G. Thiel
Neuron-specific Gene Expression of Synapsin I
J. Biol. Chem.,
February 9, 1996;
271(6):
3317 - 3323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Steiner, J. G. Hohmann, A. Holmes, C. C. Wrenn, G. Cadd, A. Jureus, D. K. Clifton, M. Luo, M. Gutshall, S. Y. Ma, et al.
Galanin transgenic mice display cognitive and neurochemical deficits characteristic of Alzheimer's disease
PNAS,
March 27, 2001;
98(7):
4184 - 4189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
