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Journal of Neuroscience, Vol 14, 2503-2514, Copyright © 1994 by Society for Neuroscience
Developmental regulation of voltage-gated K+ channel and GABAA receptor expression in Bergmann glial cells
T Muller, JM Fritschy, J Grosche, GD Pratt, H Mohler and H Kettenmann
Department of Neurobiology, University of Heidelberg, Germany.
Bergmann glial cells are closely associated with neurons: during
development they provide guiding structures for migrating granule cells and
in the adult cerebellum they display intimate interactions with Purkinje
cells. In this study, we have addressed the question of whether such
changes in neuronal-glial interactions during development are accompanied
by variations in the membrane properties of Bergmann glial cells. We used a
mouse cerebellum slice preparation to study membrane currents of the
Bergmann glial cells at various stages of development in situ using the
patch-clamp technique. The distinct morphology of Bergmann glial cells was
revealed by Lucifer yellow injections during recording. While Bergmann
glial cells in mice of postnatal day 20 (P20) to P30 have thick processes
with arborized, irregularly shaped leaf-like appendages, the processes of
cells from younger mice (P5-P7) are thinner and smoother. This
morphological maturation is accompanied by a variation in voltage-gated
currents. In cells from P5 to P7, delayed outward- and inward-rectifying K+
currents were recorded, while older Bergmann glial cells were characterized
by, large, voltage- and time-independent K+ currents. In addition,
application of GABA induces two effects, a rapid activation of a Cl-
conductance and a longer-lasting decrease in the (resting) K+ conductance.
Both effects were mediated by benzodiazepine-insensitive GABAA receptors.
Responses in cells of P5-P7 mice were large as compared to the small or
even undetectable responses in P20-P30 cells. These GABAA receptors were
characterized immunohistochemically in mice and rat brain sections with
five subunit-specific antibodies. Bergmann glial cells exhibit a distinct
but transient immunoreactivity for the GABAA receptor alpha 2-, alpha 3-,
and delta-subunits. Staining is maximal between P7 and P10 and decreases
gradually thereafter. In contrast, antibodies to the alpha 1- and beta
2,3-subunits fail to decorate Bergmann glial cells, although they yield a
prominent staining of both the Purkinje cells and the granule cells. These
changes in the Bergmann glial cell membrane properties and GABAA receptor
expression suggest a transition between functional states during
development of the Bergmann glial cells.
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