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Journal of Neuroscience, Vol 14, 2569-2578, Copyright © 1994 by Society for Neuroscience
Developmentally regulated alternative splicing generates a complex array of Drosophila para sodium channel isoforms
JR Thackeray and B Ganetzky
Laboratory of Genetics, University of Wisconsin, Madison 53706.
The para locus encodes the predominant class of sodium channels expressed
in Drosophila neurons. Previous sequence analysis of para cDNAs indicated
the occurrence of alternative splicing at several sites within the open
reading frame. Here we report a detailed analysis of this alternative
splicing and its regulation during development. We have used a combination
of RNA-PCR and sequence analysis to examine a 1.7 kilobase region of the
para mRNA that encompasses the previously reported sites of alternative
splicing. Five sites of alternative splicing were identified; 48 different
splice variants could be generated by the differential exon usage observed.
The number of splice forms and their relative frequency in vivo were
characterized in RNA samples of both embryos and adults. The range of
splice types was found to be much more diverse in adults than in embryos;
of a total of 19 different combinations of alternative exons, 11 splice
types were found in embryos and 18 in adults. Usage of some individual
alternative exons changed during development; a newly identified exon,
which is found in one of two forms either 24 or 30 base pairs long, was
present in about 85% of para transcripts from embryos but only 7% of those
in adults. These data suggest that a wide variety of subtly distinct Na
channel isoforms are present in Drosophila, and that these may provide a
range of voltage-gated sodium channel functions. Although multiple sodium
channel genes have already been described in both Drosophila and mammalian
systems, this study provides a clear indication that sodium channel
variability may be much greater than previously thought.
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