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Journal of Neuroscience, Vol 14, 2789-2799, Copyright © 1994 by Society for Neuroscience
Overexpression of a potassium channel gene perturbs neural differentiation
SM Jones and AB Ribera
Department of Physiology, University of Colorado Health Sciences Center, Denver 80262.
Functional regulation of potassium currents in developing neurons is
pivotal for changes in excitability and action potential waveform. Here, we
test whether an excess of potassium channel transcripts is sufficient to
drive functional expression of potassium current and shortening of the
duration of the action potential. Injection of Shaker- like potassium
channel transcripts into two-cell stage embryos achieves increases in RNA
levels. The elevated levels of potassium channel RNA produce larger
delayed-rectifier currents. Action potential durations are briefer,
indicating that larger potassium currents are not compensated by changes in
inward currents. Strikingly, overexpression of potassium current RNA leads
to a reduction in the number of morphologically differentiated neurons in
culture. We suggest that, by prematurely reducing the duration of the
impulse, early overexpression of potassium channel activity suppresses
normal developmental cues.
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