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Journal of Neuroscience, Vol 14, 2830-2843, Copyright © 1994 by Society for Neuroscience
Synaptic and nonsynaptic localization of the GluR1 subunit of the AMPA- type excitatory amino acid receptor in the rat cerebellum
A Baude, E Molnar, D Latawiec, RA McIlhinney and P Somogyi
Anatomical Neuropharmacology Unit, University of Oxford, United Kingdom.
The cellular and subcellular distribution of the GluR1 subunit of the
AMPA-type excitatory amino acid receptor was determined in the cerebellar
cortex of rat using immunocytochemistry. Two polyclonal antibodies were
raised against the N- and C-terminal regions of the subunit. They both
labeled a band in immunoblots of rat cerebellar membranes with a molecular
weight corresponding to that predicted for this subunit of 105 kDa
molecular mass. In light microscopy the distribution of immunoreactivity
for the two antibodies was very similar. The molecular layer was strongly
immunoreactive whereas no labeling was observed in the granular layer.
Electron microscopy revealed that the antibody raised against the
N-terminal part of the subunit recognizes an extracellular epitope(s),
whereas the antibody against the C-terminal part recognizes an
intracellular epitope(s) along the plasma membrane. In Bergmann glial cells
the endoplasmic reticulum, Golgi apparatus, and multivesicular bodies were
labeled, presumably demonstrating sites of synthesis and degradation for
the GluR1 subunit, respectively. Immunoreactivity was associated with
Bergmann glial processes surrounding Purkinje cell dendrites, spines, and
the glutamate-releasing axon terminals of the parallel and climbing fibers.
This suggests that the neurotransmitter glutamate and the AMPA- type
glutamate receptors are involved in neuronal/glial communication. The GluR1
subunit was also found at glial membranes in contact with other glial
cells. Purkinje cells showed immunoreactivity in the endoplasmic reticulum
and multivesicular bodies. No immunoreaction was detected in basket and
stellate cells. Immunoreactivity was observed at type 1 synaptic junctions,
including the synaptic cleft. These synaptic junctions were between spines,
often originating from Purkinje cell dendrites, and parallel or climbing
fiber terminals. Our results demonstrate that the GluR1 subunit of the
AMPA-type ionotropic excitatory amino acid receptor is present at both
parallel and climbing fiber synapses, which are surrounded by glial
processes containing the same receptor subunit.
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