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Journal of Neuroscience, Vol 14, 3139-3155, Copyright © 1994 by Society for Neuroscience
Identification of membrane proteins that comprise the plasmalemmal junction between migrating neurons and radial glial cells
RS Cameron and P Rakic
Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06510-8001.
We have initiated studies to identify membrane polypeptides of radial glial
cells that contribute to the selective cell-cell recognition and migration
events in developing brain. Of several polyclonal antisera evaluated, one
(D4), developed against formaldehyde fixed type 1 cerebellar glial cells,
immunolabels the free surface of cortical and cerebellar astroglial and
radial glial cells in a patchy pattern. In dissociated glial-neuronal cell
cocultures, microdomains of immunoreactivity are detected at the site where
the somal region of cells with the morphology of migrating neurons is in
contact with an elongated glial cell fiber. Microdomains are absent from
oligodendrocytes, process-bearing astrocytes, and neurons. The surface
microdomains do not colocalize with components that compose focal adhesion
plaques--integrin subunits, vinculin, or actin--and their integrity appears
to require an intact microtubule rather than actin cytoskeleton.
Furthermore, microdomain structure is maintained in the absence of
extracellular Ca and Mg ions. Immunoblot analyses using antibodies affinity
purified to individual proteins indicate that the microdomains are composed
of two antigens with apparent molecular mass of approximately 48 kDa and
approximately 72 kDa. The 48 kDa antigen is not observed in non-neural
epithelial tissues and is detected in cortical and cerebellar tissues only
at a developmental period that coincides with the stage of active neuronal
cell migration. In contrast, the 72 kDa antigen is expressed in many neural
and non-neural tissues at late developmental and adult stages. Our data
suggest that the identified membrane proteins may contribute to the
formation of the junction between migrating neurons and radial glial cell
processes and that this junctional complex is linked to the microtubule
cytoskeleton.
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