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Journal of Neuroscience, Vol 14, 3620-3627, Copyright © 1994 by Society for Neuroscience

ARTICLE

Androgenic regulation of tubulin gene expression in axotomized hamster facial motoneurons

KJ Jones and MM Oblinger
Department of Physical Therapy, University of Illinois at Chicago 60612.

We have previously demonstrated that systemic administration of testosterone increases the rate of axonal regeneration following facial nerve crush in adult male hamsters. In this investigation, the molecular mechanisms by which androgens may enhance axonal regeneration were examined. Specifically, the following question was addressed using Northern blot and in situ hybridization with three cytoskeletal cDNA probes complementary to beta II-, beta III-, and alpha 1-tubulin mRNA: does exogenous testosterone augment axotomy-induced changes in tubulin mRNA expression in hamster facial motoneurons (FMN)? Adult male hamsters were subjected to unilateral facial nerve severance, with the opposite side serving as an internal control. One-half of the animals were subcutaneously implanted with Silastic capsules containing crystalline testosterone propionate and the other half implanted with blank capsules. Postoperative survival times were 2 and 7 d. At 2 d after axotomy alone, no changes in levels of any of the three tubulin mRNAs were observed in the injured FMN. However, by 7 d after axotomy, significant increases in all three tubulin mRNAs were observed. This time course of axotomy-induced changes in tubulin gene expression is consistent with findings in other injured neuronal populations. Administration of testosterone at the time of injury had two major effects on the cytoskeletal response pattern in axotomized FMN. First, testosterone differentially regulated the set of tubulin mRNAs examined, in that beta II-tubulin mRNA levels were selectively altered by the steroid, whereas beta III- or alpha 1-tubulin mRNAs were not.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				C. B. Huppenbauer, L. Tanzer, L. L. DonCarlos, and K. J. Jones Gonadal Steroid Attenuation of Developing Hamster Facial Motoneuron Loss by Axotomy: Equal Efficacy of Testosterone, Dihydrotestosterone, and 17-{beta} Estradiol J. Neurosci., April 20, 2005; 25(16): 4004 - 4013. [Abstract] [Full Text] [PDF]

				J. Perez and D. B. Kelley Androgen Mitigates Axotomy-Induced Decreases in Calbindin Expression in Motor Neurons J. Neurosci., October 1, 1997; 17(19): 7396 - 7403. [Abstract] [Full Text] [PDF]

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