 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 3688-3703, Copyright © 1994 by Society for Neuroscience
Activity-induced changes in synaptic release sites at the crayfish neuromuscular junction
JM Wojtowicz, L Marin and HL Atwood
Department of Physiology, University of Toronto, Ontario, Canada.
Crustacean motor axons provide a model in which activity-dependent changes
in synaptic physiology and synaptic structure can be concurrently observed
in single identifiable neurons. In response to a train of stimulation,
crustacean neuromuscular junctions undergo pronounced facilitation of
transmitter release. The effects of maintained high-frequency stimulation
may persist for at least several hours ("long-term facilitation").
Electrophysiological studies suggest that the number of "active" synapses
contributing transmitter quanta at low frequencies of stimulation increases
during and after a train of high-frequency stimulation. However, at
different terminal recording sites the effect of stimulation varies, and it
was observed that not all released quanta produce a voltage change in the
postsynaptic muscle cell. Electron microscopic examinations of serial
sections from nerve terminals subjected to stimulation were made to
determine whether changes in synaptic structure could be correlated with
activity-induced long-lasting enhancement of transmission. A procedure was
introduced for marking a recorded terminal with fluorescent polystyrene
microspheres, which are visible in electron micrographs of the recording
site. Crustacean nerve terminals possess a large number of discrete
synapses, a small fraction of which have multiple presynaptic "active
zones" (dense bodies with clustered synaptic vesicles, thought to represent
sites of evoked transmitter release). In terminals previously stimulated,
the proportion of synapses with multiple "active zones" is greater than in
control unstimulated terminals. Total synaptic vesicle counts and readily
releasable vesicles at synapses are not significantly different in
previously stimulated terminals and controls. In terminals fixed during
stimulation a few synapses show evidence of division in "active zones," and
synaptic vesicle counts are lower than in controls.(ABSTRACT TRUNCATED AT
250 WORDS)
This article has been cited by other articles:
|
|
|
|
 
A. Minami, Y.-f. Xia, and R. S. Zucker
Increased Ca2+ influx through Na+/Ca2+ exchanger during long-term facilitation at crayfish neuromuscular junctions
J. Physiol.,
December 1, 2007;
585(2):
413 - 427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Kittel, C. Wichmann, T. M. Rasse, W. Fouquet, M. Schmidt, A. Schmid, D. A. Wagh, C. Pawlu, R. R. Kellner, K. I. Willig, et al.
Bruchpilot Promotes Active Zone Assembly, Ca2+ Channel Clustering, and Vesicle Release
Science,
May 19, 2006;
312(5776):
1051 - 1054.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.-L. Belair, J. Vallee, and R. Robitaille
Long-term in vivo modulation of synaptic efficacy at the neuromuscular junction of Rana pipiens frogs
J. Physiol.,
November 15, 2005;
569(1):
163 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Millar, R. S. Zucker, G. C. R. Ellis-Davies, M. P. Charlton, and H. L. Atwood
Calcium Sensitivity of Neurotransmitter Release Differs at Phasic and Tonic Synapses
J. Neurosci.,
March 23, 2005;
25(12):
3113 - 3125.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Zhai and H. J. Bellen
The Architecture of the Active Zone in the Presynaptic Nerve Terminal
Physiology,
October 1, 2004;
19(5):
262 - 270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Millar and H. L. Atwood
Crustacean Phasic and Tonic Motor Neurons
Integr. Comp. Biol.,
February 1, 2004;
44(1):
4 - 13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Sigrist, D. F. Reiff, P. R. Thiel, J. R. Steinert, and C. M. Schuster
Experience-Dependent Strengthening of Drosophila Neuromuscular Junctions
J. Neurosci.,
July 23, 2003;
23(16):
6546 - 6556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Millar, H. Bradacs, M. P. Charlton, and H. L. Atwood
Inverse Relationship between Release Probability and Readily Releasable Vesicles in Depressing and Facilitating Synapses
J. Neurosci.,
November 15, 2002;
22(22):
9661 - 9667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. F. Reiff, P. R. Thiel, and C. M. Schuster
Differential Regulation of Active Zone Density during Long-Term Strengthening of Drosophila Neuromuscular Junctions
J. Neurosci.,
November 1, 2002;
22(21):
9399 - 9409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Sigrist, P. R. Thiel, D. F. Reiff, and C. M. Schuster
The Postsynaptic Glutamate Receptor Subunit DGluR-IIA Mediates Long-Term Plasticity in Drosophila
J. Neurosci.,
September 1, 2002;
22(17):
7362 - 7372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. D. Gover, X.-Y. Jiang, and T. W. Abrams
Persistent, Exocytosis-Independent Silencing of Release Sites Underlies Homosynaptic Depression at Sensory Synapses in Aplysia
J. Neurosci.,
March 1, 2002;
22(5):
1942 - 1955.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Crider and R. L. Cooper
Differential facilitation of high- and low-output nerve terminals from a single motoneuron
J Appl Physiol,
March 1, 2000;
88(3):
987 - 996.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. L. Atwood and J. M. Wojtowicz
Silent Synapses in Neural Plasticity: Current Evidence
Learn. Mem.,
November 1, 1999;
6(6):
542 - 571.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Msghina, A. G. Millar, M. P. Charlton, C. K. Govind, and H. L. Atwood
Calcium Entry Related to Active Zones and Differences in Transmitter Release at Phasic and Tonic Synapses
J. Neurosci.,
October 1, 1999;
19(19):
8419 - 8434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bykhovskaia, J. T. Hackett, and M. K. Worden
Asynchrony of Quantal Events in Evoked Multiquantal Responses Indicates Presynaptic Quantal Interaction
J Neurophysiol,
May 1, 1999;
81(5):
2234 - 2242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-i. Morishige, A. Inanobe, Y. Yoshimoto, H. Kurachi, Y. Murata, Y. Tokunaga, T. Maeda, Y. Maruyama, and Y. Kurachi
Secretagogue-induced Exocytosis Recruits G Protein-gated K+ Channels to Plasma Membrane in Endocrine Cells
J. Biol. Chem.,
March 19, 1999;
274(12):
7969 - 7974.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Quigley, M. Msghina, C. K. Govind, and H. L. Atwood
Visible Evidence for Differences in Synaptic Effectiveness With Activity-Dependent Vesicular Uptake and Release of FM1-43
J Neurophysiol,
January 1, 1999;
81(1):
356 - 370.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Feinstein, D. Parnas, H. Parnas, J. Dudel, and I. Parnas
Functional and Immunocytochemical Identification of Glutamate Autoreceptors of an NMDA Type in Crayfish Neuromuscular Junction
J Neurophysiol,
December 1, 1998;
80(6):
2893 - 2899.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Msghina, C. K. Govind, and H. L. Atwood
Synaptic Structure and Transmitter Release in Crustacean Phasic and Tonic Motor Neurons
J. Neurosci.,
February 15, 1998;
18(4):
1374 - 1382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Kabotyanski, D. A. Baxter, and J. H. Byrne
Identification and Characterization of Catecholaminergic Neuron B65, Which Initiates and Modifies Patterned Activity in the Buccal Ganglia of Aplysia
J Neurophysiol,
February 1, 1998;
79(2):
605 - 621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Martinez-Dunst, R. L. Michaels, and P. A. Fuchs
Release Sites and Calcium Channels in Hair Cells of the Chick's Cochlea
J. Neurosci.,
December 1, 1997;
17(23):
9133 - 9144.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Chen and W. G. Regehr
The Mechanism of cAMP-Mediated Enhancement at a Cerebellar Synapse
J. Neurosci.,
November 15, 1997;
17(22):
8687 - 8694.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Worden, M. Bykhovskaia, and J. T. Hackett
Facilitation at the Lobster Neuromuscular Junction: A Stimulus-Dependent Mobilization Model
J Neurophysiol,
July 1, 1997;
78(1):
417 - 428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhu, F. Wu, and S. Schacher
Site-Specific and Sensory Neuron-Dependent Increases in Postsynaptic Glutamate Sensitivity Accompany Serotonin-Induced Long-Term Facilitation at Aplysia Sensorimotor Synapses
J. Neurosci.,
July 1, 1997;
17(13):
4976 - 4986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Caroni, L. Aigner, and C. Schneider
Intrinsic Neuronal Determinants Locally Regulate Extrasynaptic and Synaptic Growth at the Adult Neuromuscular Junction
J. Cell Biol.,
February 10, 1997;
136(3):
679 - 692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Passafaro, P. Rosa, C. Sala, F. Clementi, and E. Sher
N-type Ca2+ Channels Are Present in Secretory Granules and Are Transiently Translocated to the Plasma Membrane during Regulated Exocytosis
J. Biol. Chem.,
November 22, 1996;
271(47):
30096 - 30104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R F Rogers and L D Matzel
Higher-order associative processing in Hermissenda suggests multiple sites of neuronal modulation.
Learn. Mem.,
January 1, 1996;
2(6):
279 - 298.
[Abstract]
[PDF]
|
|
|
|
