 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 3922-3933, Copyright © 1994 by Society for Neuroscience
Comparison of mammalian adult and fetal nicotinic acetylcholine receptors stably expressed in fibroblasts
C Kopta and JH Steinbach
Department of Anesthesiology, Washington University School of Medicine, St. Louis, Missouri 63130.
Cells from a line of transformed quail fibroblasts (QT-6) were transfected
with cDNAs coding for subunits of the mouse muscle nicotinic ACh receptor
(AChR). Stable clones were selected that expressed subunits of the
fetal-type AChR (alpha, beta, gamma, delta) or the adult-type AChR (alpha,
beta, epsilon, delta). The receptors had the appropriate burst durations
and single-channel conductances for the fetal or adult type, respectively.
Each type of receptor had a dose- response relationship that was close to a
square law at low concentrations of ACh, implying that they contained two
ACh-binding subunits. The metabolic stability of surface fetal and adult
receptors was identical (about 10 hr half-life), for two independent clones
expressing fetal and two expressing adult AChR. The metabolic stability was
unaffected by treatment with okadaic acid, which enhanced receptor
phosphorylation. d-Tubocurarine (dTC) blocked both the binding of alpha-
bungarotoxin (BTX) to the cells and the ACh-elicited current. dTC blocked
BTX binding with indistinguishable efficacy for both fetal and adult AChR.
However, it was sixfold less effective at blocking ACh- elicited current
from fetal AChR. At least part of the difference results from the ability
of fetal receptor channels to open when the receptor has one ACh and one
dTC molecule bound, whereas channels of heteroliganded adult receptors do
not open. The data indicate that the subunit composition directly affects
physiological and pharmacological properties of muscle AChR, but has little
effect by itself on metabolic stability.
This article has been cited by other articles:
|
|
|
|
 
G. Akk and J. H. Steinbach
Galantamine Activates Muscle-Type Nicotinic Acetylcholine Receptors without Binding to the Acetylcholine-Binding Site
J. Neurosci.,
February 23, 2005;
25(8):
1992 - 2001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Herzig and J. Neumann
Effects of Serine/Threonine Protein Phosphatases on Ion Channels in Excitable Membranes
Physiol Rev,
January 1, 2000;
80(1):
173 - 210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kurata, W. Marszalec, J. Z. Yeh, and T. Narahashi
Agonist and Potentiation Actions of n-Octanol on gamma -Aminobutyric Acid Type A Receptors
Mol. Pharmacol.,
June 1, 1999;
55(6):
1011 - 1019.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Z.-Z. Wang, A. Mathias, M. Gautam, and Z. W. Hall
Metabolic Stabilization of Muscle Nicotinic Acetylcholine Receptor by Rapsyn
J. Neurosci.,
March 15, 1999;
19(6):
1998 - 2007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Grassi, O. Epifano, A. M. Mileo, B. Barabino, and F. Eusebi
The open duration of fetal ACh receptor-channel changes during mouse muscle development
J. Physiol.,
April 15, 1998;
508(2):
393 - 400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J Maconochie and J. H. Steinbach
The channel opening rate of adult- and fetal-type mouse muscle nicotinic receptors activated by acetylcholine
J. Physiol.,
January 1, 1998;
506(1):
53 - 72.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Sala, J. O'Malley, R. Xu, G. Fumagalli, and M. M. Salpeter
epsilon Subunit-Containing Acetylcholine Receptors in Myotubes Belong to the Slowly Degrading Population
J. Neurosci.,
December 1, 1997;
17(23):
8937 - 8944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Pena and S. G. Rane
The Small Conductance Calcium-activated Potassium Channel Regulates Ion Channel Expression in C3H10T1/2 Cells Ectopically Expressing the Muscle Regulatory Factor MRF4
J. Biol. Chem.,
August 29, 1997;
272(35):
21909 - 21916.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. O'Malley, C. T. Moore, and M. M. Salpeter
Stabilization of Acetylcholine Receptors by Exogenous ATP and Its Reversal by cAMP and Calcium
J. Cell Biol.,
July 14, 1997;
138(1):
159 - 165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Missias, J Mudd, J. Cunningham, J. Steinbach, J. Merlie, and J. Sanes
Deficient development and maintenance of postsynaptic specializations in mutant mice lacking an 'adult' acetylcholine receptor subunit
Development,
January 12, 1997;
124(24):
5075 - 5086.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. Xie and J. B. Cohen
Contributions of Torpedo Nicotinic Acetylcholine Receptor gamma Trp-55 and delta Trp-57 to Agonist and Competitive Antagonist Function
J. Biol. Chem.,
January 19, 2001;
276(4):
2417 - 2426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
