QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miller, P. D. Articles by DeKosky, S. T. Search for Related Content PubMed PubMed Citation Articles by Miller, P. D. Articles by DeKosky, S. T.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 14, 4217-4225, Copyright © 1994 by Society for Neuroscience

ARTICLE

Embryonic neural cell adhesion molecule (N-CAM) is elevated in the denervated rat dentate gyrus

PD Miller, SD Styren, CF Lagenaur and ST DeKosky
Department of Neurobiology, University of Pittsburgh School of Medicine, Pennsylvania.

We evaluated the immunohistological changes in neural cell adhesion molecule (N-CAM) expression in the adult rat dentate gyrus during the period of synaptic degeneration, axonal sprouting, and synaptogenesis following ipsilateral entorhinal cortex (ERC) lesion. This lesion denervates the outer two-thirds of the dentate granule cells dendrites and induces compensatory sprouting from the subjacent inner one-third into the denervated zone, as well as reactive synaptogenesis in the denervated outer molecular layer. In unlesioned adult hippocampus, antibodies to total N-CAM stained the inner molecular layer intensely, and the outer molecular layer (ML) more lightly. After ERC lesion the intense staining of the inner layer widened, the expansion following the known temporal sequence of commissural and associational (C/A) axon sprouting into the denervated zone. In normal unlesioned controls there was very light, uniform staining of the ML with antibodies directed against embryonic N-CAM (eN-CAM). By 2 d post-ERC lesion, the outer two- thirds of the ML stained robustly with antibody to eN-CAM. This area of intense staining receded as the C/A axon collaterals from the inner one- third entered the denervated zone, so that by 30 d the intense eN-CAM staining only occupied the outer half of the ML. The increased expression of eN-CAM remained present at 60 d post-ERC lesion, past the point that synaptic volume density has returned to normal levels in the denervated zone. Ultrastructural studies showed that the newly expressed eN-CAM was located on the surface of dendrites in the denervated zone, but was not found at the synaptic contacts.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				Y. Sato, Y. Akimoto, H. Kawakami, H. Hirano, and T. Endo Location of Sialoglycoconjugates Containing the Sia{alpha}2-3Gal and Sia{alpha}2-6Gal Groups in the Rat Hippocampus and the Effect of Aging on Their Expression J. Histochem. Cytochem., October 1, 2001; 49(10): 1311 - 1320. [Abstract] [Full Text] [PDF]

				C. A. Haas, U. Rauch, N. Thon, T. Merten, and T. Deller Entorhinal Cortex Lesion in Adult Rats Induces the Expression of the Neuronal Chondroitin Sulfate Proteoglycan Neurocan in Reactive Astrocytes J. Neurosci., November 15, 1999; 19(22): 9953 - 9963. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help