 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 4244-4251, Copyright © 1994 by Society for Neuroscience
Differential regulation of the release of the same peptide transmitters from individual identified motor neurons in culture
MD Whim and PE Lloyd
Department of Pharmacological and Physiological Sciences, University of Chicago, Illinois 60637.
Aplysia motor neurons B1, B2, and B15 synthesize the small cardioactive
peptides A and B (SCPs). In previous studies using semi-intact preparations
we have demonstrated that the SCPs are released from B15. Significant
peptide release only occurred when B15 was stimulated at a high frequency
or at lower frequencies with a long burst duration. In the behaving animal
B15 fires in patterns expected to release the SCPs. In contrast, in the
behaving animal, neurons B1 and B2 fire at much lower frequencies. We
therefore examined whether similar aspects of the stimulation pattern
governed the release of the SCPs from B1 and B2. To monitor peptide
release, all three neurons were individually cultured and newly synthesized
peptides labeled with 35S-methionine. Release of labeled SCPs was detected
by HPLC of extracts of superfusates. By keeping spike number constant and
varying the stimulation pattern, the release of the SCPs from B1 and B2 was
found to be pattern insensitive. That is, regardless of the stimulation
paradigm, each action potential released a similar amount of peptide. By
severing the primary neurite, peptide release was found to occur mainly
from the regenerated neurites and most likely from the intensely
immunoreactive varicosites. Calcium- and stimulation-dependent release of
the SCPs and a third neuropeptide termed buccalin A from motor neuron B15
was also observed in culture. The release of these peptides from B15 was
found to be pattern sensitive as was observed in the semi-intact
preparations.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
|
|
 
A. Balkowiec and D. M. Katz
Cellular Mechanisms Regulating Activity-Dependent Release of Native Brain-Derived Neurotrophic Factor from Hippocampal Neurons
J. Neurosci.,
December 1, 2002;
22(23):
10399 - 10407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-K. Ng, X. Lu, S. C. Watkins, G. C. R. Ellis-Davies, and E. S. Levitan
Nerve Growth Factor-Induced Differentiation Changes the Cellular Organization of Regulated Peptide Release by PC12 Cells
J. Neurosci.,
May 15, 2002;
22(10):
3890 - 3897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Fox and P. E. Lloyd
Evidence That Post-Tetanic Potentiation Is Mediated by Neuropeptide Release in Aplysia
J Neurophysiol,
December 1, 2001;
86(6):
2845 - 2855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Hurwitz, E. C. Cropper, F. S. Vilim, V. Alexeeva, A. J. Susswein, I. Kupfermann, and K. R. Weiss
Serotonergic and Peptidergic Modulation of the Buccal Mass Protractor Muscle (I2) in Aplysia
J Neurophysiol,
December 1, 2000;
84(6):
2810 - 2820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Balkowiec and D. M. Katz
Activity-Dependent Release of Endogenous Brain-Derived Neurotrophic Factor from Primary Sensory Neurons Detected by ELISA In Situ
J. Neurosci.,
October 1, 2000;
20(19):
7417 - 7423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Brezina, P. J. Church, and K. R. Weiss
Temporal Pattern Dependence of Neuronal Peptide Transmitter Release: Models and Experiments
J. Neurosci.,
September 15, 2000;
20(18):
6760 - 6772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. T. Morgan, R. Perrins, P. E. Lloyd, and K. R. Weiss
Intrinsic and Extrinsic Modulation of a Single Central Pattern Generating Circuit
J Neurophysiol,
September 1, 2000;
84(3):
1186 - 1193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. S. Vilim, E. C. Cropper, D. A. Price, I. Kupfermann, and K. R. Weiss
Peptide Cotransmitter Release from Motorneuron B16 in Aplysia californica: Costorage, Corelease, and Functional Implications
J. Neurosci.,
March 1, 2000;
20(5):
2036 - 2042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Brezina, I. V. Orekhova, and K. R. Weiss
Control of time-dependent biological processes by temporally patterned input
PNAS,
September 16, 1997;
94(19):
10444 - 10449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Whim, H. Niemann, and L. K. Kaczmarek
The Secretion of Classical and Peptide Cotransmitters from a Single Presynaptic Neuron Involves a Synaptobrevin-Like Molecule
J. Neurosci.,
April 1, 1997;
17(7):
2338 - 2347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
