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Journal of Neuroscience, Vol 14, 4600-4612, Copyright © 1994 by Society for Neuroscience
Ionic currents of Kenyon cells from the mushroom body of the honeybee
S Schafer, H Rosenboom and R Menzel
Institut fur Neurobiologie, Freien Universitat, Berlin, Germany.
The mushroom bodies have been suggested to be essentially involved in
learning and memory in insects. In the honeybee Apis mellifera they are
composed of about 340,000 intrinsic elements, called Kenyon cells, which
can be easily separated from all other neurons of the brain. Here we
describe a preparation in which we studied ionic currents in the isolated
Kenyon cell somata, using tight-seal whole-cell recording. Several outward
and inward currents were identified and investigated by the use of
pharmacological agents and in ion substitution experiments: a rapidly
inactivating A-type potassium current that is completely blocked with 5 mM
4-aminopyridine; a calcium-activated potassium current that is blocked by
1-100 nM charybdotoxin; a delayed rectifier- type potassium current that is
only weakly sensitive to tetraethylammonium but is blocked by 100 microM
quinidine; a rapidly activating and inactivating, TTX-sensitive sodium
current; a persistent sodium current that is both TTX and cadmium
sensitive; and a calcium current that is completely blocked at 50 microM
cadmium and is affected by verapamil and nifedipine only at high
concentrations (100 microM). The currents described here are very similar
to currents found in other insect neurons or muscle cells. This preparation
will not only facilitate studies concerning the action of transmitters and
neuromodulators that are contained within neurons converging onto the
Kenyon cells, but will also allow a study of the role of the adenylyl
cyclase pathway, elements of which are expressed in Kenyon cells, and are
known to be essential for learning in invertebrates.
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