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Journal of Neuroscience, Vol 14, 4613-4638, Copyright © 1994 by Society for Neuroscience
Detailed passive cable models of whole-cell recorded CA3 pyramidal neurons in rat hippocampal slices
G Major, AU Larkman, P Jonas, B Sakmann and JJ Jack
University Laboratory of Physiology, Oxford, United Kingdom.
Tight-seal whole-cell recordings were made from cleaned somata of CA3
pyramidal cells deep in hippocampal slices from 19-21-d-old rats. The cells
were filled with biocytin, and their voltage responses to short current
pulses were recorded. After washout of initial sag, responses scaled
linearly with injected current and were stable over time. The dendritic and
axonal arbors of four cells were reconstructed and measured using light
microscopy. Dendritic spines and axonal boutons were counted and the
additional membrane area was incorporated into the relevant segments. The
morphology of each neuron was converted into a detailed branching cable
model by assuming values for specific membrane capacitance Cm and
resistance Rm, and cytoplasmic resistivity Ri. These parameters were
optimized for each cell by directly matching the model's response to that
of the real cell by means of a modified weighted least-squares fitting
procedure. By comparing the deviations between model and experimental
responses to control noise recordings, approximate 95% confidence intervals
were established for each parameter. If a somatic shunt was allowed, a wide
range of possible Rm values produced acceptable fits. With zero shunt, Cm
was 0.7-0.8 microFcm-2, Ri was 170-340 omega cm, and Rm ranged between 120
and 200 k omega cm2. The electrotonic lengths of the basal and oblique
dendrites were 0.2-0.3 space constants, and those of the apical tufts were
0.4-0.7 space constants. The steady-state electrical geometry of these
cells was therefore compact; average dendritic tip/soma relative synaptic
efficacies were > 93% for the basal and oblique dendrites, and > 81%
for the tufts. With fast transient synaptic inputs, however, the models
produced a wide range of postsynaptic potential shapes and marked filtering
of voltage-clamp currents.
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