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Journal of Neuroscience, Vol 14, 4639-4655, Copyright © 1994 by Society for Neuroscience
Regional differences in glial-derived factors that promote dendritic outgrowth from mouse cortical neurons in vitro
PD Le Roux and TA Reh
Department of Neurosurgery, University of Washington, Seattle 98195.
To determine whether glia from different CNS regions differ in their
ability to support axons or dendrites, embryonic (E18) mouse cortical
neurons were cocultured with early postnatal (P4) rat astroglial derived
from cortex, retina, olfactory bulb, mesencephalon, striatum, and spinal
cord. After 5 d in vitro, axon and dendrite outgrowth from isolated neurons
was quantified with double-labeling immunohistochemical techniques. Whereas
axonal growth was similar on the various monolayers, total primary
dendritic outgrowth was nearly threefold greater on glia derived from the
cortex, retina, and olfactory bulb than on glia derived from mesencephalon,
striatum, or spinal cord. This effect was principally on the number of
primary dendrites rather than the elongation of individual dendrites.
Similar morphological differences were observed when cortical neurons were
grown on polylysine in a noncontact coculture system with glia continuously
conditioning the media. This selective promotion of dendrite growth was
independent of neuron survival. These results indicate that there are
regional differences in the ability of CNS glia to support dendritic growth
and that this effect is due, in part, to release of a diffusable factor.
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