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Journal of Neuroscience, Vol 14, 4825-4830, Copyright © 1994 by Society for Neuroscience
Induction of c-fos expression in hypothalamic magnocellular neurons requires synaptic activation and not simply increased spike activity
SM Luckman, RE Dyball and G Leng
Department of Neurobiology, AFRC Babraham Institute, Cambridge, United Kingdom.
Magnocellular neurons of the hypothalamic supraoptic nucleus have been
shown to express the immediate-early gene c-fos in a number of experimental
and physiological circumstances. In each case the induction of the
immediate-early gene followed the increase in the spike activity of the
cells. Since an increase in the intracellular concentration of calcium
following influx through voltage-sensitive calcium channels is a known
stimulus for c-fos expression and since the action potentials of these
neurons have a large calcium component, we hypothesized that c-fos
induction in these neurons could be attributed to calcium influx during
spike activity. In the present experiments we use extracellular recording
and immunocytochemistry for Fos, the protein product of c-fos, to
demonstrate the activation of the cells following intracerebroventricular
administration of the muscarinic agonist, carbachol. Fos expression
following carbachol injection was then compared with that induced by a
similar number of antidromically evoked action potentials. Antidromic
activation, unlike the activation induced by carbachol, did not lead to the
induction of Fos. We conclude that Fos induction in these neurons requires
receptor activation rather than spike activity.
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