WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience The New Axio Examiner
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Usui, H.
Right arrow Articles by Sutcliffe, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Usui, H.
Right arrow Articles by Sutcliffe, J. G.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 14, 4915-4926, Copyright © 1994 by Society for Neuroscience

ARTICLE

Isolation of clones of rat striatum-specific mRNAs by directional tag PCR subtraction

H Usui, JD Falk, A Dopazo, L de Lecea, MG Erlander and JG Sutcliffe
Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037.

We report an improved subtractive cDNA cloning procedure, named "directional tag PCR subtraction," for isolating clones of mRNAs enriched in a target tissue compared to a second tissue, the driver. In this method, the target and driver are prepared from directional cDNA libraries constructed in different vectors, and the target cDNA contains tag sequences at both its 5' and 3' ends for PCR amplification. This method avoids several limitations of previous subtraction procedures, and was demonstrated to be technically easy and efficient. Using the directional tag PCR subtraction and improved screening procedures, cDNA clones corresponding to mRNAs expressed in the striatum but not in the cerebellum of the rat brain were efficiently isolated, including mRNAs encoding calmodulin-dependent phosphodiesterase, a transcriptional regulatory protein, and several previously uncharacterized species. Our data suggest that approximately 1% of the striatal polyA+ RNA mass potentially encoding more than 300 distinct proteins corresponds to RNA species reduced in concentration or absent from the cerebellum, of which about one-third are expressed prominently only in the striatum. This unexpected finding suggests that the striatum has a unique biochemical character within the brain, and that characterization of these mRNAs will be important for understanding the biochemical basis of striatal function.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-W. Chang, C.-W. Tsai, H.-F. Wang, H.-C. Tsai, H.-Y. Chen, T.-F. Tsai, H. Takahashi, H.-Y. Li, M.-J. Fann, C.-W. Yang, et al.
Identification of a developmentally regulated striatum-enriched zinc-finger gene, Nolz-1, in the mammalian brain
PNAS, February 24, 2004; 101(8): 2613 - 2618.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. de Chaldee, M.-C. Gaillard, N. Bizat, J.-M. Buhler, O. Manzoni, J. Bockaert, P. Hantraye, E. Brouillet, and J.-M. Elalouf
Quantitative Assessment of Transcriptome Differences Between Brain Territories
Genome Res., July 1, 2003; 13(7): 1646 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. E. Alvarez, J. G. Sutcliffe, and E. A. Thomas
Novel Isoform of Insulin Receptor Substrate p53/p58 Is Generated by Alternative Splicing in the CRIB/SH3-binding Region
J. Biol. Chem., June 28, 2002; 277(27): 24728 - 24734.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. G. Sutcliffe
Open-System Approaches to Gene Expression in the CNS
J. Neurosci., November 1, 2001; 21(21): 8306 - 8309.
[Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
P. K. Vogt, M. Aoki, I. Bottoli, H.-W. Chang, S.-l. Fu, A. Hecht, J. S. Iacovoni, B.-H. Jiang, and U. Kruse
A Random Walk in Oncogene Space: The Quest for Targets
Cell Growth Differ., December 1, 1999; 10(12): 777 - 784.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S.-l. Fu, I. Bottoli, M. Goller, and P. K. Vogt
Heparin-binding epidermal growth factor-like growth factor, a v-Jun target gene, induces oncogenic transformation
PNAS, May 11, 1999; 96(10): 5716 - 5721.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Kawasaki, G. M. Springett, S. Toki, J. J. Canales, P. Harlan, J. P. Blumenstiel, E. J. Chen, I. A. Bany, N. Mochizuki, A. Ashbacher, et al.
A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia
PNAS, October 27, 1998; 95(22): 13278 - 13283.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P Ruiz-Lozano, S. Smith, G Perkins, S. Kubalak, G. Boss, H. Sucov, R. Evans, and K. Chien
Energy deprivation and a deficiency in downstream metabolic target genes during the onset of embryonic heart failure in RXRalpha-/- embryos
Development, January 2, 1998; 125(3): 533 - 544.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
R. W. Williams, R. C. Strom, D. S. Rice, and D. Goldowitz
Genetic and Environmental Control of Variation in Retinal Ganglion Cell Number in Mice
J. Neurosci., November 15, 1996; 16(22): 7193 - 7205.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. V. Nguyen, H. Fujioka, A. S. Kang, W. O. Rogers, D. A. Fidock, and A. A. James
Stage-dependent Localization of a Novel Gene Product of the Malaria Parasite, Plasmodium falciparum
J. Biol. Chem., July 6, 2001; 276(28): 26724 - 26731.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-