 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 4998-5010, Copyright © 1994 by Society for Neuroscience
Pathway-specific variants of AMPA receptors and their contribution to neuronal signaling
IM Raman, S Zhang and LO Trussell
Neuroscience Training Program, University of Wisconsin-Madison 53706.
Neurons of the nucleus magnocellularis (nMAG) of the chick express AMPA
(alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate) receptors
displaying unusually rapid kinetics of desensitization (Raman and Trussell,
1992a). To investigate whether fast AMPA receptors are present in other
auditory neurons, we compared the properties of AMPA receptors in auditory
as well as nonauditory cells. These included neurons of nMAG, the nucleus
angularis, the nucleus laminaris, the cochlear ganglion, the Purkinje cell
layer of the cerebellum, the ventral horn of the spinal cord, and the
brainstem nucleus of the glossopharyngeal nerve (ncIX). Rapid application
of glutamate to voltage-clamped outside-out membrane patches indicated that
AMPA receptors in the four types of auditory cells had significantly faster
kinetics of desensitization than did the three types of nonauditory
neurons. Channel kinetics in auditory (nMAG) and nonauditory (ncIX) cells
were also compared by means of spectral analysis and the time course of
current deactivation upon removal of glutamate. Both techniques revealed a
burst duration for nMAG channels of 400-500 musec at room temperature, two
to three times shorter than for ncIX cells. Single channels in nMAG had a
burst duration of 550 musec and an open time of approximately 150 musec.
Miniature excitatory postsynaptic currents of brainstem auditory neurons in
slices were also brief, with decay constants of 150-250 musec at 29-32
degrees C. We demonstrated that the fast kinetics of this AMPA receptor are
physiologically important, since cyclothiazide, which reduces
desensitization and prolongs synaptic currents, doubled the relative
refractory period of orthodromic spikes in nMAG cells in brain slices. We
conclude that auditory brainstem neurons express a specialized subtype of
AMPA receptors. This "fast" AMPA receptor may be useful in transmitting
signals necessary for sound localization.
This article has been cited by other articles:
|
|
|
|
 
D. A. DiGregorio, J. S. Rothman, T. A. Nielsen, and R. A. Silver
Desensitization Properties of AMPA Receptors at the Cerebellar Mossy Fiber Granule Cell Synapse
J. Neurosci.,
August 1, 2007;
27(31):
8344 - 8357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Szabo, S. A. Weiss, D. S. Faber, and T. Preuss
Representation of Auditory Signals in the M-Cell: Role of Electrical Synapses
J Neurophysiol,
April 1, 2006;
95(4):
2617 - 2629.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K Magnusson, C. Kapfer, B. Grothe, and U. Koch
Maturation of glycinergic inhibition in the gerbil medial superior olive after hearing onset
J. Physiol.,
October 15, 2005;
568(2):
497 - 512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. K. Dasika, J. A. White, L. H. Carney, and H. S. Colburn
Effects of Inhibitory Feedback in a Network Model of Avian Brain Stem
J Neurophysiol,
July 1, 2005;
94(1):
400 - 414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. MacLeod and C. E. Carr
Synaptic Physiology in the Cochlear Nucleus Angularis of the Chick
J Neurophysiol,
May 1, 2005;
93(5):
2520 - 2529.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Joshi, S. Shokralla, P. Titis, and L.-Y. Wang
The Role of AMPA Receptor Gating in the Development of High-Fidelity Neurotransmission at the Calyx of Held Synapse
J. Neurosci.,
January 7, 2004;
24(1):
183 - 196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Lawrence, S. Brenowitz, and L. O. Trussell
The Mechanism of Action of Aniracetam at Synaptic {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors: Indirect and Direct Effects on Desensitization
Mol. Pharmacol.,
August 1, 2003;
64(2):
269 - 278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Y. C. Wong, B. P. Graham, B. Billups, and I. D. Forsythe
Distinguishing between Presynaptic and Postsynaptic Mechanisms of Short-Term Depression during Action Potential Trains
J. Neurosci.,
June 15, 2003;
23(12):
4868 - 4877.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Xu-Friedman and W. G. Regehr
Ultrastructural Contributions to Desensitization at Cerebellar Mossy Fiber to Granule Cell Synapses
J. Neurosci.,
March 15, 2003;
23(6):
2182 - 2192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Soares, R. A. Chitwood, R. L. Hyson, and C. E. Carr
Intrinsic Neuronal Properties of the Chick Nucleus Angularis
J Neurophysiol,
July 1, 2002;
88(1):
152 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Gardner, L. O. Trussell, and D. Oertel
Correlation of AMPA Receptor Subunit Composition with Synaptic Input in the Mammalian Cochlear Nuclei
J. Neurosci.,
September 15, 2001;
21(18):
7428 - 7437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Vandenberghe, E. C. Ihle, D. K. Patneau, W. Robberecht, and J. R. Brorson
AMPA Receptor Current Density, Not Desensitization, Predicts Selective Motoneuron Vulnerability
J. Neurosci.,
October 1, 2000;
20(19):
7158 - 7166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zirpel, M. A. Janowiak, C. A. Veltri, and T. N. Parks
AMPA Receptor-Mediated, Calcium-Dependent CREB Phosphorylation in a Subpopulation of Auditory Neurons Surviving Activity Deprivation
J. Neurosci.,
August 15, 2000;
20(16):
6267 - 6275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Lawrence and L. O. Trussell
Long-Term Specification of AMPA Receptor Properties after Synapse Formation
J. Neurosci.,
July 1, 2000;
20(13):
4864 - 4870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Monsivais, L. Yang, and E. W Rubel
GABAergic Inhibition in Nucleus Magnocellularis: Implications for Phase Locking in the Avian Auditory Brainstem
J. Neurosci.,
April 15, 2000;
20(8):
2954 - 2963.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Moser and D. Beutner
Kinetics of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse of the mouse
PNAS,
January 18, 2000;
97(2):
883 - 888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Gardner, L. O. Trussell, and D. Oertel
Time Course and Permeation of Synaptic AMPA Receptors in Cochlear Nuclear Neurons Correlate with Input
J. Neurosci.,
October 15, 1999;
19(20):
8721 - 8729.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Carvalho, K. Kameyama, and R. L. Huganir
Characterization of Phosphorylation Sites on the Glutamate Receptor 4 Subunit of the AMPA Receptors
J. Neurosci.,
June 15, 1999;
19(12):
4748 - 4754.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yang, P. Monsivais, and E. W Rubel
The Superior Olivary Nucleus and Its Influence on Nucleus Laminaris: A Source of Inhibitory Feedback for Coincidence Detection in the Avian Auditory Brainstem
J. Neurosci.,
March 15, 1999;
19(6):
2313 - 2325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-X. Wang, R. J. Wenthold, O. P. Ottersen, and R. S. Petralia
Endbulb Synapses in the Anteroventral Cochlear Nucleus Express a Specific Subset of AMPA-Type Glutamate Receptor Subunits
J. Neurosci.,
February 1, 1998;
18(3):
1148 - 1160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Ferragamo, N. L. Golding, and D. Oertel
Synaptic Inputs to Stellate Cells in the Ventral Cochlear Nucleus
J Neurophysiol,
January 1, 1998;
79(1):
51 - 63.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sekiguchi, M. W. Fleck, M. L. Mayer, J. Takeo, Y. Chiba, S. Yamashita, and K. Wada
A Novel Allosteric Potentiator of AMPA Receptors: 4-[2-(Phenylsulfonylamino)ethylthio]-2,6-Difluoro-Phenoxyacetamide
J. Neurosci.,
August 1, 1997;
17(15):
5760 - 5771.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-M. Zhou and J. J. Hablitz
Rapid Kinetics and Inward Rectification of Miniature EPSCs in Layer I Neurons of Rat Neocortex
J Neurophysiol,
May 1, 1997;
77(5):
2416 - 2426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kuba, K. Koyano, and H. Ohmori
Development of membrane conductance improves coincidence detection in the nucleus laminaris of the chicken
J. Physiol.,
April 15, 2002;
540(2):
529 - 542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
