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 Previous Article
Journal of Neuroscience, Vol 14, 5131-5146, Copyright © 1994 by Society for Neuroscience
Glycinergic synapses in the rod pathway of the rat retina: cone bipolar cells express the alpha 1 subunit of the glycine receptor
M Sassoe-Pognetto, H Wassle and U Grunert
Max-Planck-Institut fur Hirnforschung, Neuroanatomische Abteilung, Frankfurt am Main, Germany.
Glycine receptors (GlyRs) and their role in retinal circuitry were analyzed
immunocytochemically in the rat retina. Specific antibodies against the
alpha 1 subunit of the GlyR and against the GlyR-associated protein
gephyrin, respectively, were used. In the inner plexiform layer (IPL), both
antibodies produced a punctate label that was shown by electron microscopy
to occur at synapses. Gephyrin-like immunoreactivity (-LI) was more widely
distributed, indicating that gephyrin might also occur at nonglycinergic
synapses. At the ultrastructural level, gephyrin-LI was found at the
cytoplasmic face of postsynaptic membranes of amacrine and ganglion cells,
but was never detected in bipolar cell axons. Immunoreactivity for the
alpha 1 subunit was concentrated in the cleft of conventional synapses made
by amacrine cell processes onto ganglion cell dendrites and cone bipolar
axons. The latter synapses differ from other glycinergic synapses since
they are not labeled by the antibody against gephyrin used in this study.
In order to identify the type of bipolar cell involved in these synapses,
the distribution of the alpha 1 subunit was compared with that of
recoverin-immunoreactive cone bipolar cells and with that of
parvalbumin-immunoreactive All-amacrine cells. Double-label
immunofluorescence showed that, in the outer part of the IPL, 75% of the
alpha 1-immunoreactive puncta were colocalized with recoverin- positive
bipolar cell axons and 71% of the alpha 1-immunoreactive puncta were
colocalized with parvalbumin-positive All-amacrine processes. Hence, the
alpha 1 subunit of the GlyR is present at the chemical synapses established
by All-amacrine cells with OFF-cone bipolar cells and OFF-ganglion cells.
These synapses play a key role in the transmission of scotopic signals
through the OFF-channel of the rod pathway.
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