QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hong, C. S. Articles by Ganetzky, B. Search for Related Content PubMed PubMed Citation Articles by Hong, C. S. Articles by Ganetzky, B.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 14, 5160-5169, Copyright © 1994 by Society for Neuroscience

ARTICLE

Spatial and temporal expression patterns of two sodium channel genes in Drosophila

CS Hong and B Ganetzky
Laboratory of Genetics, University of Wisconsin, Madison 53706.

Genetic and molecular studies have identified two different sodium channel genes in Drosophila, para and DSC1. The functional contributions of the para-encoded channel have been inferred from analysis of mutant phenotypes. However, no mutations of DSC1 have been identified, so the in vivo functions of the channel it encodes are not yet known. To learn more about the possible functions of DSC1 in the Drosophila nervous system compared with those of para, we have characterized the expression patterns of these two sodium channel genes at embryonic, larval, pupal, and adult stages by tissue in situ hybridization, para encodes the predominant type of sodium channel and is ubiquitously transcribed throughout the CNS and PNS at all developmental stages. The expression pattern of DSC1 is very different from para during embryonic and larval stages during which there are very few DSC1-expressing cells in either the CNS or PNS. Double- labeling studies suggest that some of these cells are non-neuronal. However, in pupal and adult stages, para and DSC1 have completely overlapping patterns of expression in the CNS and retina. In the pupal and adult, PNS expression of these genes is still distinct because only para transcripts are detected in wing sensory neurons. The strong and widespread expression of DSC1 in the CNS of pupae and adults suggests that the DSC1 channels are likely to provide an important function in neurons during these stages. Since most, if not all, neurons in the pupal and adult CNS express both para and DSC1, these two sodium channel genes probably subserve distinct functions within these cells. Our results provide the background for elucidating the respective in vivo contributions of para and DSC1 to neuronal excitability and for dissecting the regulatory mechanisms that underlie their different patterns of expression.

This article has been cited by other articles:

				H. A. Lindsay, R. Baines, R. ffrench-Constant, K. Lilley, H. T. Jacobs, and K. M. C. O'Dell The Dominant Cold-Sensitive Out-Cold Mutants of Drosophila melanogaster Have Novel Missense Mutations in the Voltage-Gated Sodium Channel Gene paralytic Genetics, October 1, 2008; 180(2): 873 - 884. [Abstract] [Full Text] [PDF]

				J. Lee and C.-F. Wu Genetic Modifications of Seizure Susceptibility and Expression by Altered Excitability in Drosophila Na+ and K+ Channel Mutants J Neurophysiol, November 1, 2006; 96(5): 2465 - 2478. [Abstract] [Full Text] [PDF]

				C. J. Mee, E. C. G. Pym, K. G. Moffat, and R. A. Baines Regulation of Neuronal Excitability through Pumilio-Dependent Control of a Sodium Channel Gene J. Neurosci., October 6, 2004; 24(40): 8695 - 8703. [Abstract] [Full Text] [PDF]

				E. S. Montana and J. T. Littleton Characterization of a hypercontraction-induced myopathy in Drosophila caused by mutations in Mhc J. Cell Biol., March 29, 2004; 164(7): 1045 - 1054. [Abstract] [Full Text] [PDF]

				N. H. Kulkarni, A. H. Yamamoto, K. O. Robinson, T. F. C. Mackay, and R. R. H. Anholt The DSC1 Channel, Encoded by the smi60E Locus, Contributes to Odor-Guided Behavior in Drosophila melanogaster Genetics, August 1, 2002; 161(4): 1507 - 1516. [Abstract] [Full Text] [PDF]

				H. A. Nash In vivo genetics of anaesthetic action Br. J. Anaesth., July 1, 2002; 89(1): 143 - 155. [Abstract] [Full Text] [PDF]

				G. F. Lopreato, Y. Lu, A. Southwell, N. S. Atkinson, D. M. Hillis, T. P. Wilcox, and H. H. Zakon Evolution and divergence of sodium channel genes in vertebrates PNAS, June 19, 2001; 98(13): 7588 - 7592. [Abstract] [Full Text] [PDF]

				D. Wicher Peptidergic Modulation of an Insect Na+ Current: Role of Protein Kinase A and Protein Kinase C J Neurophysiol, January 1, 2001; 85(1): 374 - 383. [Abstract] [Full Text] [PDF]

				L. Yuan and B. Ganetzky A Glial-Neuronal Signaling Pathway Revealed by Mutations in a Neurexin-Related Protein Science, February 26, 1999; 283(5406): 1343 - 1345. [Abstract] [Full Text]

				S. A. Titus, J. W. Warmke, and B. Ganetzky The Drosophila erg K+ Channel Polypeptide Is Encoded by the Seizure Locus J. Neurosci., February 1, 1997; 17(3): 875 - 881. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help