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Journal of Neuroscience, Vol 14, 5243-5256, Copyright © 1994 by Society for Neuroscience
Three novel types of voltage-dependent calcium channels in rat cerebellar neurons
L Forti, A Tottene, A Moretti and D Pietrobon
Department of Biomedical Sciences, University of Padova, Italy.
With the aim of characterizing the functional and pharmacological
properties of the different voltage-dependent Ca2+ channels expressed in a
given type of CNS neuron, we obtained single Ca2+ channel recordings from
rat cerebellar granule cells in primary culture. Our data show that three
novel classes of voltage-dependent Ca2+ channels are coexpressed in
cerebellar granule cells. They are pharmacologically distinct from
dihydropyridine-sensitive L-type and omega-conotoxin- sensitive N-type
channels, and their functional properties are different from those of P-
and T-type channels. The three novel 21 pS G1-, 15 pS G2-, and 20 pS
G3-type Ca2+ channels have similar inactivation properties. They show
complete steady-state inactivation at -40 mV and their single-channel
average currents have both sustained and decaying components. They differ
in activation threshold (-40 mV for G2, -30 mV for G3, and -10 mV for G1,
with 90 mM Ba2+ as charge carrier), mean open time (1.2 msec for G2, 1 msec
for G3, 0.8 msec for G1), and single-channel currents (at 0 mV: 0.5 pA for
G2, 0.8 pA for G3, and 1.4 pA for G1). Together with the previously
characterized multiple L-type Ca2+ channels (Forti and Pietrobon, 1993),
G1-, G2-, and G3-type channels constitute the large majority of Ca2+
channels of cerebellar granule cells in culture. The low activation
threshold of G2- type channels and their inactivation properties suggest
that they might be native counterparts of the recently expressed rat brain
clone rbE-II (Soong et al., 1993).
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