WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience behavioral testing systems
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barry, E. L.
Right arrow Articles by Froehner, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barry, E. L.
Right arrow Articles by Froehner, S. C.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 15, 274-283, Copyright © 1995 by Society for Neuroscience

ARTICLE

Expression and antibody inhibition of P-type calcium channels in human small-cell lung carcinoma cells

EL Barry, MP Viglione, YI Kim and SC Froehner
Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03755.

P-type channels, a recently described form of voltage-gated calcium channels, are found in many central and peripheral neurons. In the present study, a partial cDNA clone sharing extensive nucleotide identity with a putative P-type voltage-gated calcium channel alpha 1 subunit was isolated from a small-cell lung carcinoma (SCLC) cell line. Anti-peptide antibodies generated to a unique acidic stretch in the IVS5-S6 linker region of the putative SCLC P-type channel reacted specifically with a SCLC fusion protein produced in bacteria and with a cell surface molecule in SCLC cells. Calcium currents in SCLC cells, measured by whole-cell patch clamp, were inhibited by these antibodies and by the P-type channel-specific toxin omega-agatoxin IVA. The inhibitory effects of the antibody and the toxin were not additive, consistent with their proposed action on the same type of channel. These results provide evidence for the expression of P-type calcium channels by SCLC cells. The expression of neuron-related molecules by these cells is of particular interest because small-cell lung carcinoma is frequently associated with paraneoplastic disorders affecting the nervous system.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. J. Liao, P. Safa, Y.-R. Chen, R. A. Sobel, E. S. Boyden, and R. W. Tsien
Anti-Ca2+ channel antibody attenuates Ca2+ currents and mimics cerebellar ataxia in vivo
PNAS, February 19, 2008; 105(7): 2705 - 2710.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y.-F. Xu, S. J. Hewett, and W. D. Atchison
Passive Transfer of Lambert-Eaton Myasthenic Syndrome Induces Dihydropyridine Sensitivity of ICa in Mouse Motor Nerve Terminals
J Neurophysiol, September 1, 1998; 80(3): 1056 - 1069.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-