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Journal of Neuroscience, Vol 15, 298-309, Copyright © 1995 by Society for Neuroscience

ARTICLE

Forskolin and camptothecin induce a 30 kDa protein associated with melatonin production in Y79 human retinoblastoma cells

JL Janavs, JC Florez, ME Pierce and JS Takahashi
Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208-3520.

The synthesis of melatonin in Xenopus retinas, chick and quail retinal cell cultures, and Y79 human retinoblastoma cells is stimulated by cAMP through a protein synthesis-dependent mechanism. In Y79 retinoblastoma cells, combined treatment with the RNA synthesis inhibitor camptothecin and agents that elevate cAMP, such as forskolin, causes a synergistic elevation of melatonin. Using two-dimensional gel analysis we have identified a 30 kDa cytosolic protein (p30) whose radiolabeling was consistently increased in parallel with increases in arylalkylamine N- acetyltransferase activity and melatonin production that were induced by forskolin and/or camptothecin. Pulse-chase experiments suggest that the elevation in radiolabeling of p30 is due to increased synthesis. Three candidate proteins found in the mammalian pineal, protein 14-3-3, malate dehydrogenase, and recoverin, do not comigrate with p30.


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 J Biol RhythmsHome page
J. C. Florez and J. S. Takahashi
Quantitative Two-Dimensional Gel Electrophoretic Analysis of Clock-Controlled Proteins in Cultured Chick Pineal Cells: Circadian Regulation of Tryptophan Hydroxylase
J Biol Rhythms, September 1, 1996; 11(3): 241 - 257.
[Abstract] [PDF]


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