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Journal of Neuroscience, Vol 15, 394-406, Copyright © 1995 by Society for Neuroscience
Developmental and growth factor-induced regulation of nestin in oligodendrocyte lineage cells
V Gallo and RC Armstrong
Laboratory of Cellular and Molecular Neurophysiology, NICHD, NIH, Bethesda, Maryland 20892.
The expression and regulation of nestin, an intermediate filament protein
associated with neuroepithelium-derived progenitor cells, was studied in
developing oligodendrocytes. We analyzed glial cells cultured from late
embryonic rat cerebral cortex, tissue prints from neonatal rat brains, and
the oligodendrocyte cell line CG-4. Using cortical- or CG-4-derived
oligodendrocyte lineage cells, Northern blot analysis demonstrated that
nestin mRNA was highly expressed in proliferating O-2A progenitors, but
downregulated in differentiated oligodendrocytes. Immunocytochemistry of
cultured cells and tissue prints demonstrated that pre-O-2A cells and O-2A
progenitors expressed high levels of the nestin protein. In contrast,
nestin expression decreased during the pro-oligodendroblast stage, and the
majority of oligodendrocytes were not labeled with anti-nestin antibodies.
Culture conditions (PDGF+bFGF or B104-conditioned medium) that maintained
O-2A progenitor proliferation and prevented differentiation enhanced
expression of nestin mRNA transcripts and protein. In comparison, analysis
of cortical astrocyte cultures demonstrated that type 1 astrocytes, but not
type 2, expressed nestin mRNA and protein. Our findings demonstrate that
nestin is expressed in developing glial cells, which is suggestive that
this intermediate filament protein may play an important role during
gliogenesis.
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