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Journal of Neuroscience, Vol 15, 562-573, Copyright © 1995 by Society for Neuroscience
CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system
Z Kaprielian, KO Cho, M Hadjiargyrou and PH Patterson
Biology Division, California Institute of Technology, Pasadena 91125.
We previously generated a monoclonal antibody (mAb), ROCA1, which binds
preferentially to rostral versus caudal sympathetic ganglia and intercostal
nerves. Two other mAbs, ROCA2 and B2C11, bind to the same structures but
not in rostrocaudal gradients. All three mAbs recognize a 26 kDa cell
surface protein. Amino acid sequence data obtained from the affinity
purified 26 kDa protein showed some homology with human CD9, a tetraspan
protein implicated in intercellular signaling in hematopoietic cells. Using
the PCR, we obtained cDNA clones representing the entire rat CD9 coding
sequence from sciatic nerve and sympathetic ganglia. ROCA1, ROCA2, and
B2C11 each immunoprecipitate a 26 kDa protein from CHO cells stably
transfected with one of the clones, demonstrating that the ROCA cell
surface antigen is indeed rat CD9. We find that CD9 mRNA is widely
expressed, with particularly high levels present in a number of neural
tissues. In situ hybridization demonstrates that peripheral neurons and
Schwann cells, as well as adrenal chromaffin cells express CD9 mRNA.
Consistent with immunoblot analyses showing that, unlike the ROCA1 epitope,
the 26 kDa protein is not expressed in a rostrocaudal gradient, we find
similar levels of rat CD9 mRNA in rostral and caudal intercostal nerves. In
developing postnatal rat sciatic nerve, CD9 mRNA levels are coordinately
regulated with the expression of myelin genes. These results provide
another example of a cell surface protein expressed by both hematopoietic
and neural cells, and suggest a role for CD9 in intercellular signaling in
the nervous system.
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