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Journal of Neuroscience, Vol 15, 6351-6363, Copyright © 1995 by Society for Neuroscience
Cerebellar precursors transplanted to the neonatal dentate gyrus express features characteristic of hippocampal neurons
C Vicario-Abejon, MG Cunningham and RD McKay
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge 02139, USA.
During the development of the CNS, a salient issue is whether neuronal
phenotype is defined by the lineage or by the environment of precursor
cells. Transplants permit these two possibilities to be tested, as cell
fate can be examined in a new location. Dissociated cerebellar cells from
newborn rats treated with tritiated thymidine or from NSE-lacZ transgenic
mice were grafted into the dentate gyrus of the developing hippocampus.
Implanted cells integrated into the granule cell layer, which contains the
cell bodies of host granule neurons. Immunohistochemistry showed that
grafted cells in the granule cell layer, like the host hippocampal granule
neurons, were calbindin positive and upregulated FOS in a seizure paradigm.
Electron microscopic analysis also showed that cells grafted to the dentate
gyrus share features with host dentate neurons. These assays indicate that
transplanted cerebellar cells acquired morphological and antigenic features
characteristic of hippocampal neurons. These results show that
metencephalic precursors are capable of differentiating in response to
signals in the telencephalon, suggesting that the environment controls the
regional fate of neuronal precursor cells during neurogenesis.
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