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Journal of Neuroscience, Vol 15, 6433-6444, Copyright © 1995 by Society for Neuroscience
Role of calcium channel subtypes in calcium transients in hippocampal CA3 neurons
EM Elliott, AT Malouf and WA Catterall
Department of Pharmacology, University of Washington, Seattle 98195, USA.
Multiple subtypes of voltage-gated calcium channels are differentially
localized in brain neurons suggesting that they serve distinct roles in
neuronal excitation and signaling. In organotypic hippocampal slice
cultures, class D (L-type) calcium channels are predominantly located in
the cell bodies of CA3 neurons while class B (N-type) and class A (P or
Q-type) are localized in dendrites and associated presynaptic terminals
with relatively low somal expression. Using specific antagonists to inhibit
calcium transients recorded in CA3 neuronal cell bodies, we found that
L-type calcium channels have a predominant role in somal calcium transients
elicited by trains of strong stimuli applied to either the soma or the
distal apical dendrite while class A calcium channels make a smaller
contribution. Presynaptic class B (N- type) and class A (P- and/or Q-type)
calcium channels are critical for glutamate-mediated synaptic transmission
onto the dendrites of CA3 neurons. Postsynaptic class A and B calcium
channels detected on the dendritic shaft by immunocytochemistry were not
found to contribute substantially to somal calcium transients during
repetitive stimulation of distal dendrites, but sodium channels were
required for calcium transients elicited by somatic or dendritic
stimulation. Our results show that the different calcium channel subtypes
serve distinct roles in cellular activation and transmission of signals in
CA3 neurons, consistent with their differential subcellular localization.
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