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Journal of Neuroscience, Vol 15, 7238-7249, Copyright © 1995 by Society for Neuroscience
Early patterns of migration, morphogenesis, and intermediate filament expression of subventricular zone cells in the postnatal rat forebrain
M Zerlin, SW Levison and JE Goldman
Department of Pathology, Columbia University College of P&S, New York, New York 10032, USA.
Recent studies using retroviral labeling of subventricular zone (SVZ)
progenitors in vivo in neonatal rats have directly demonstrated the
generation of both astrocytes and oligodendrocytes from these progenitors.
In the present study, we used a recombinant retroviral vector encoding
beta-galactosidase, and analyzed brains within the first week after
retroviral injection to trace the early routes that SVZ cells take as they
migrate into white matter and cortex and characterized the early
morphological and antigenic changes that accompanied their differentiation.
SVZ cells follow specifically definable migratory routes as they colonize
the cortex and subcortical white matter. Glial progenitors do not populate
the cortex in a systematic, laminar fashion, as do neuroblasts. The
abundance of labeled progenitors in radial arrangements and the close
apposition of many immature cells to vimentin+ radial glial processes,
suggest that glial progenitors migrate along radial glia. Labeled SVZ
cells, which displayed a simple, unipolar or bipolar morphology, lacked
detectable vimentin and nestin intermediate filaments. Similarly, beta-
galactosidase-positive cells in white matter lacked these filaments. In
contrast, labeled, multipolar cells in the cortex, and a few of the
immature-appearing cortical cells expressed nestin and vimentin. At these
early time points, GFAP was not detected in beta-galactosidase- labeled
cells. Multipolar cells in cortex frequently displayed processes extending
toward and contacting blood vessels. These observations suggest that the
expression of nestin and vimentin occurs after progenitors emigrate from
the SVZ and that filament expression and contact with blood vessels
represent an early stage of astrocyte differentiation.
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